ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage

@article{citeulike:1352803, abstract = {Cellular responses to DNA damage are mediated by a number of protein kinases, including ATM (ataxia telangiectasia mutated) and ATR (ATM and Rad3-related). The outlines of the signal transduction portion of this pathway are known, but little is known about the physiological scope of the DNA damage response (DDR). We performed a large-scale proteomic analysis of proteins phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR and identified more than 900 regulated phosphorylation sites encompassing over 700 proteins. Functional analysis of a subset of this data set indicated that this list is highly enriched for proteins involved in the DDR. This set of proteins is highly interconnected, and we identified a large number of protein modules and networks not previously linked to the DDR. This database paints a much broader landscape for the DDR than was previously appreciated and opens new avenues of investigation into the responses to DNA damage in mammals. 10.1126/science.1140321}, author = {Matsuoka, Shuhei and Ballif, Bryan A. and Smogorzewska, Agata and Mcdonald, Robert E. and Hurov, Kristen E. and Luo, Ji and Bakalarski, Corey E. and Zhao, Zhenming and Solimini, Nicole and Lerenthal, Yaniv and Shiloh, Yosef and Gygi, Steven P. and Elledge, Stephen J. }, citeulike-article-id = {1352803}, doi = {http://dx.doi.org/10.1126/science.1140321}, journal = {Science}, keywords = {atm, atr, dna-damage, phosphorylation}, month = {May}, number = {5828}, pages = {1160--1166}, posted-at = {2007-12-20 22:37:35}, priority = {2}, title = {ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage}, url = {http://dx.doi.org/10.1126/science.1140321}, volume = {316}, year = {2007} }

TY - JOUR ID - citeulike:1352803 L3 - citeulike-article-id:1352803 TI - ATM and ATR Substrate Analysis Reveals Extensive Protein Networks Responsive to DNA Damage JF - Science VL - 316 IS - 5828 SP - 1160 EP - 1166 N2 - Cellular responses to DNA damage are mediated by a number of protein kinases, including ATM (ataxia telangiectasia mutated) and ATR (ATM and Rad3-related). The outlines of the signal transduction portion of this pathway are known, but little is known about the physiological scope of the DNA damage response (DDR). We performed a large-scale proteomic analysis of proteins phosphorylated in response to DNA damage on consensus sites recognized by ATM and ATR and identified more than 900 regulated phosphorylation sites encompassing over 700 proteins. Functional analysis of a subset of this data set indicated that this list is highly enriched for proteins involved in the DDR. This set of proteins is highly interconnected, and we identified a large number of protein modules and networks not previously linked to the DDR. This database paints a much broader landscape for the DDR than was previously appreciated and opens new avenues of investigation into the responses to DNA damage in mammals. 10.1126/science.1140321 KW - atm KW - atr KW - dna-damage KW - phosphorylation AU - Matsuoka, Shuhei AU - Ballif, Bryan AU - Smogorzewska, Agata AU - Mcdonald, Robert AU - Hurov, Kristen AU - Luo, Ji AU - Bakalarski, Corey AU - Zhao, Zhenming AU - Solimini, Nicole AU - Lerenthal, Yaniv AU - Shiloh, Yosef AU - Gygi, Steven AU - Elledge, Stephen PY - 2007/05/25/ UR - http://dx.doi.org/10.1126/science.1140321 ER -