Single-cell c-myc gene expression in relationship to nuclear domains

@article{citeulike:2759057, abstract = {Nuclear locations of the c-myc gene and its transcripts (c-myc T) have been investigated in relation to nuclear domains involved in RNA synthesis and processing. Transcription of the c-myc gene appears to be linked to the late G1- and preferentially to S-phases of the cell cycle. The c-myc gene and its transcripts were positioned non-randomly within the interphase nucleus; additionally, c-myc RNA signals accumulated at nucleoli. Using oligo-probes, designed to exon II and exon III of the c-myc gene, single c-myc T was preferentially observed in human carcinoma HT29 and A549 cells. Conversely, human embryonal teratocarcinoma NTERA cells were characterized by the presence of multiple c-myc RNA signals located in both the nucleoli and nucleoplasm. When accumulated at nucleoli, c-myc T occupied the periphery of this organelle, though not those associated with the cultivation surface. In HT29 cells, approximately 80\% of c-myc T co-localized with the RNAP II positive regions, so-called transcription factories. However, in ?20\% of the cells with c-myc transcripts, the c-myc T was released from the site of synthesis, and was not associated with either transcription factories or SC35 domains. In ?60\% of nuclei with c-myc T, these signals were located in close proximity to the SC35 regions, but promyelocytic leukaemia bodies were associated with c-myc T only in ?20\% of the nuclei. Taken together, c-myc RNA signals were positioned in the most internal parts of the cell nuclei preferentially associated with the nucleoli. Specific nuclear and nucleolar positioning probably reflects the kinetics of c-myc RNA metabolism. {\copyright} 2008 Springer.}, address = {Institute of Biophysics, Academy of Sciences of the Czech Republic, Kra?lovopolska? 135, CZ-612 65 Brno, Czech Republic}, author = {Ba?rtova?, E. and Harnic?arova?, A. and Krejc?i?, J. and Stras?a?k, L. and Kozubek, S. }, citeulike-article-id = {2759057}, doi = {10.1007/s10577-007-1196-0}, journal = {Chromosome Research}, keywords = {myc, nuclear\_localization}, number = {2}, pages = {325--343}, posted-at = {2008-05-05 20:38:50}, priority = {2}, title = {Single-cell c-myc gene expression in relationship to nuclear domains}, url = {http://dx.doi.org/10.1007/s10577-007-1196-0}, volume = {16}, year = {2008} }

TY - JOUR ID - citeulike:2759057 L3 - citeulike-article-id:2759057 TI - Single-cell c-myc gene expression in relationship to nuclear domains JF - Chromosome Research VL - 16 IS - 2 SP - 325 EP - 343 AD - Institute of Biophysics, Academy of Sciences of the Czech Republic, Kra?lovopolska? 135, CZ-612 65 Brno, Czech Republic N2 - Nuclear locations of the c-myc gene and its transcripts (c-myc T) have been investigated in relation to nuclear domains involved in RNA synthesis and processing. Transcription of the c-myc gene appears to be linked to the late G1- and preferentially to S-phases of the cell cycle. The c-myc gene and its transcripts were positioned non-randomly within the interphase nucleus; additionally, c-myc RNA signals accumulated at nucleoli. Using oligo-probes, designed to exon II and exon III of the c-myc gene, single c-myc T was preferentially observed in human carcinoma HT29 and A549 cells. Conversely, human embryonal teratocarcinoma NTERA cells were characterized by the presence of multiple c-myc RNA signals located in both the nucleoli and nucleoplasm. When accumulated at nucleoli, c-myc T occupied the periphery of this organelle, though not those associated with the cultivation surface. In HT29 cells, approximately 80% of c-myc T co-localized with the RNAP II positive regions, so-called transcription factories. However, in ?20% of the cells with c-myc transcripts, the c-myc T was released from the site of synthesis, and was not associated with either transcription factories or SC35 domains. In ?60% of nuclei with c-myc T, these signals were located in close proximity to the SC35 regions, but promyelocytic leukaemia bodies were associated with c-myc T only in ?20% of the nuclei. Taken together, c-myc RNA signals were positioned in the most internal parts of the cell nuclei preferentially associated with the nucleoli. Specific nuclear and nucleolar positioning probably reflects the kinetics of c-myc RNA metabolism. © 2008 Springer. KW - myc KW - nuclear_localization AU - Ba?rtova?, E AU - Harnic?arova?, A AU - Krejc?i?, J AU - Stras?a?k, L AU - Kozubek, S PY - 2008/// UR - http://dx.doi.org/10.1007/s10577-007-1196-0 ER -