Increase in nuclear poly(A)-containing RNA at syncytial blastoderm in Drosophila melanogaster embryos

@article{citeulike:1584550, abstract = {Levels of poly(A)-containing RNA in embryonic nuclei of Drosophila melanogaster were related to the morphological events of nuclear migration and cell formation. Poly(A)-containing RNA was detected by in situ hybridization of [3H]poly(U) to cytological preparations of ruptured embryos. Increased levels of [3H]poly(U) binding were observed in nuclei that had migrated to the cortical cytoplasm. Ribonuclease-treated preparations indicated that increased poly(U) binding was RNA dependent. Pole cells, which are the first cells formed, did not show this increase in binding. We interpret these data as evidence that transcriptional activation of informational sequences occurs shortly after nuclei have migrated to the cortical cytoplasm and before cell membranes are completed. The absence of this activation in nuclei of pole cells may result from repressor activity in the polar plasm.}, author = {Lamb, Mary M. and Laird, Charles D. }, citeulike-article-id = {1584550}, doi = {http://dx.doi.org/10.1016/0012-1606(76)90004-X}, journal = {Developmental Biology}, keywords = {drosophila, transcription}, month = {August}, number = {1}, pages = {31--42}, posted-at = {2007-08-23 01:53:01}, priority = {2}, title = {Increase in nuclear poly(A)-containing RNA at syncytial blastoderm in Drosophila melanogaster embryos}, url = {http://dx.doi.org/10.1016/0012-1606(76)90004-X}, volume = {52}, year = {1976} }

TY - JOUR ID - citeulike:1584550 L3 - citeulike-article-id:1584550 TI - Increase in nuclear poly(A)-containing RNA at syncytial blastoderm in Drosophila melanogaster embryos JF - Developmental Biology VL - 52 IS - 1 SP - 31 EP - 42 N2 - Levels of poly(A)-containing RNA in embryonic nuclei of Drosophila melanogaster were related to the morphological events of nuclear migration and cell formation. Poly(A)-containing RNA was detected by in situ hybridization of [3H]poly(U) to cytological preparations of ruptured embryos. Increased levels of [3H]poly(U) binding were observed in nuclei that had migrated to the cortical cytoplasm. Ribonuclease-treated preparations indicated that increased poly(U) binding was RNA dependent. Pole cells, which are the first cells formed, did not show this increase in binding. We interpret these data as evidence that transcriptional activation of informational sequences occurs shortly after nuclei have migrated to the cortical cytoplasm and before cell membranes are completed. The absence of this activation in nuclei of pole cells may result from repressor activity in the polar plasm. KW - drosophila KW - transcription AU - Lamb, Mary AU - Laird, Charles PY - 1976/08// UR - http://dx.doi.org/10.1016/0012-1606(76)90004-X ER -