Impact of maternal nutrition during pregnancy on pituitary gonadotrophin gene expression and ovarian development in growth-restricted and normally grown late gestation sheep fetuses

@article{citeulike:2532409, abstract = {The influence of maternal nutrition during pregnancy on anterior pituitary gonadotrophin gene expression and ovarian development in sheep fetuses during late gestation was investigated. Embryos recovered from superovulated adult ewes that had been inseminated by a single sire were transferred, singly, into the uteri of adolescent recipients. After embryo transfer, adolescent ewes were offered a high or moderate amount of a complete diet. Pregnancies were terminated at day 131 +/- 0.6 of gestation and the fetal brain, anterior pituitary gland and gonads were collected. Gonadotrophin gene expression (LHbeta and FSHbeta subunits) in the fetal pituitary gland was examined using in situ hybridization. Ovarian follicular development was quantified in haematoxylin- and eosin-stained ovarian sections embedded in paraffin wax. Six dams that were offered a high nutrient intake carried normal-sized fetuses (weight within +/- 2 SD of mean weight for control fetuses from dams fed a moderate level of complete diet) and 13 dams carried growth-restricted fetuses (weight 10.1530/rep.0.1230769}, author = {Da Silva, P. and Aitken, R. P. and Rhind, S. M. and Racey, P. A. and Wallace, J. M. }, citeulike-article-id = {2532409}, doi = {http://dx.doi.org/10.1530/rep.0.1230769}, journal = {Reproduction}, keywords = {expression, gene, gonadotrophin, maternal, nutrition, pituitary}, month = {June}, number = {6}, pages = {769--777}, posted-at = {2008-03-14 14:27:12}, priority = {2}, title = {Impact of maternal nutrition during pregnancy on pituitary gonadotrophin gene expression and ovarian development in growth-restricted and normally grown late gestation sheep fetuses}, url = {http://dx.doi.org/10.1530/rep.0.1230769}, volume = {123}, year = {2002} }

TY - JOUR ID - citeulike:2532409 L3 - citeulike-article-id:2532409 TI - Impact of maternal nutrition during pregnancy on pituitary gonadotrophin gene expression and ovarian development in growth-restricted and normally grown late gestation sheep fetuses JF - Reproduction VL - 123 IS - 6 SP - 769 EP - 777 N2 - The influence of maternal nutrition during pregnancy on anterior pituitary gonadotrophin gene expression and ovarian development in sheep fetuses during late gestation was investigated. Embryos recovered from superovulated adult ewes that had been inseminated by a single sire were transferred, singly, into the uteri of adolescent recipients. After embryo transfer, adolescent ewes were offered a high or moderate amount of a complete diet. Pregnancies were terminated at day 131 +/- 0.6 of gestation and the fetal brain, anterior pituitary gland and gonads were collected. Gonadotrophin gene expression (LHbeta and FSHbeta subunits) in the fetal pituitary gland was examined using in situ hybridization. Ovarian follicular development was quantified in haematoxylin- and eosin-stained ovarian sections embedded in paraffin wax. Six dams that were offered a high nutrient intake carried normal-sized fetuses (weight within +/- 2 SD of mean weight for control fetuses from dams fed a moderate level of complete diet) and 13 dams carried growth-restricted fetuses (weight 10.1530/rep.0.1230769 KW - expression KW - gene KW - gonadotrophin KW - maternal KW - nutrition KW - pituitary AU - Da Silva, P AU - Aitken, RP AU - Rhind, SM AU - Racey, PA AU - Wallace, JM PY - 2002/06/01/ UR - http://dx.doi.org/10.1530/rep.0.1230769 ER -