The Spread of Ras Activity Triggered by Activation of a Single Dendritic Spine

@article{citeulike:2910862, abstract = {In neurons, individual dendritic spines isolate NMDA receptor-mediated Ca2+ accumulations from the dendrite and other spines. However, it is not known to what extent spines compartmentalize signaling events downstream of Ca2+ influx. We combined two-photon fluorescence lifetime imaging (FLIM) with two-photon glutamate uncaging to image the activity of the small GTPase Ras following NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca2+-dependent Ras activation in single spines that decayed in approximately 5 minutes. Ras activity spread over approximately 10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus Ca2+-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite. 10.1126/science.1159675}, author = {Harvey, Christopher D. and Yasuda, Ryohei and Zhong, Haining and Svoboda, Karel }, citeulike-article-id = {2910862}, doi = {http://dx.doi.org/10.1126/science.1159675}, journal = {Science}, keywords = {090708, dendritic\_spines, ras, synaptic\_plasticity}, month = {June}, pages = {1159675+}, posted-at = {2008-07-07 12:10:06}, priority = {2}, title = {The Spread of Ras Activity Triggered by Activation of a Single Dendritic Spine}, url = {http://dx.doi.org/10.1126/science.1159675}, year = {2008} }

TY - JOUR ID - citeulike:2910862 L3 - citeulike-article-id:2910862 TI - The Spread of Ras Activity Triggered by Activation of a Single Dendritic Spine JF - Science SP - 1159675 N2 - In neurons, individual dendritic spines isolate NMDA receptor-mediated Ca2+ accumulations from the dendrite and other spines. However, it is not known to what extent spines compartmentalize signaling events downstream of Ca2+ influx. We combined two-photon fluorescence lifetime imaging (FLIM) with two-photon glutamate uncaging to image the activity of the small GTPase Ras following NMDA receptor activation at individual spines. Induction of long-term potentiation (LTP) triggered robust Ca2+-dependent Ras activation in single spines that decayed in approximately 5 minutes. Ras activity spread over approximately 10 micrometers of dendrite and invaded neighboring spines by diffusion. The spread of Ras-dependent signaling was necessary for the local regulation of the threshold for LTP induction. Thus Ca2+-dependent synaptic signals can spread to couple multiple synapses on short stretches of dendrite. 10.1126/science.1159675 KW - 090708 KW - dendritic_spines KW - ras KW - synaptic_plasticity AU - Harvey, Christopher AU - Yasuda, Ryohei AU - Zhong, Haining AU - Svoboda, Karel PY - 2008/06/12/ UR - http://dx.doi.org/10.1126/science.1159675 ER -