Imaging of an Early Memory Trace in the Drosophila Mushroom Body

@article{citeulike:2731217, abstract = {Extensive molecular, genetic, and anatomical analyses have suggested that olfactory memory is stored in the mushroom body (MB), a higher-order olfactory center in the insect brain. The MB comprises three subtypes of neurons with axons that extend into different lobes. A recent functional imaging study has revealed a long-term memory trace manifested as an increase in the Ca2+ activity in an axonal branch of a subtype of MB neurons. However, early memory traces in the MB remain elusive. We report here learning-induced changes in Ca2+ activities during early memory formation in a different subtype of MB neurons. We used three independent in vivo and in vitro preparations, and all of them showed that Ca2+ activities in the axonal branches of {alpha}'/' neurons in response to a conditioned olfactory stimulus became larger compared with one that was not conditioned. The changes were dependent on proper G-protein signaling in the MB. The importance of these changes in the Ca2+ activity of {alpha}'/' neurons during early memory formation was further tested behaviorally by disrupting G-protein signaling in these neurons or blocking their synaptic outputs during the learning and memory process. Our results suggest that increased Ca2+ activity in response to a conditioned olfactory stimulus may be a neural correlate of early memory in the MB. 10.1523/JNEUROSCI.2958-07.2008}, author = {Wang, Yalin and Mamiya, Akira and Chiang, Ann-Shyn and Zhong, Yi }, citeulike-article-id = {2731217}, doi = {http://dx.doi.org/10.1523/JNEUROSCI.2958-07.2008}, journal = {J. Neurosci.}, keywords = {290408, ca\_channels, olfaction, synaptic\_plasticity}, month = {April}, number = {17}, pages = {4368--4376}, posted-at = {2008-04-28 23:42:16}, priority = {2}, title = {Imaging of an Early Memory Trace in the Drosophila Mushroom Body}, url = {http://dx.doi.org/10.1523/JNEUROSCI.2958-07.2008}, volume = {28}, year = {2008} }

TY - JOUR ID - citeulike:2731217 L3 - citeulike-article-id:2731217 TI - Imaging of an Early Memory Trace in the Drosophila Mushroom Body JF - J. Neurosci. VL - 28 IS - 17 SP - 4368 EP - 4376 N2 - Extensive molecular, genetic, and anatomical analyses have suggested that olfactory memory is stored in the mushroom body (MB), a higher-order olfactory center in the insect brain. The MB comprises three subtypes of neurons with axons that extend into different lobes. A recent functional imaging study has revealed a long-term memory trace manifested as an increase in the Ca2+ activity in an axonal branch of a subtype of MB neurons. However, early memory traces in the MB remain elusive. We report here learning-induced changes in Ca2+ activities during early memory formation in a different subtype of MB neurons. We used three independent in vivo and in vitro preparations, and all of them showed that Ca2+ activities in the axonal branches of {alpha}'/' neurons in response to a conditioned olfactory stimulus became larger compared with one that was not conditioned. The changes were dependent on proper G-protein signaling in the MB. The importance of these changes in the Ca2+ activity of {alpha}'/' neurons during early memory formation was further tested behaviorally by disrupting G-protein signaling in these neurons or blocking their synaptic outputs during the learning and memory process. Our results suggest that increased Ca2+ activity in response to a conditioned olfactory stimulus may be a neural correlate of early memory in the MB. 10.1523/JNEUROSCI.2958-07.2008 KW - 290408 KW - ca_channels KW - olfaction KW - synaptic_plasticity AU - Wang, Yalin AU - Mamiya, Akira AU - Chiang, Ann-Shyn AU - Zhong, Yi PY - 2008/04/23/ UR - http://dx.doi.org/10.1523/JNEUROSCI.2958-07.2008 ER -