RNA Helicase A Interacts with RISC in Human Cells and Functions in RISC Loading.

@article{citeulike:1343812, abstract = {RNA interference is a conserved pathway of sequence-specific gene silencing that depends on small guide RNAs and the action of proteins assembled in the RNA-induced silencing complex (RISC). Minimally, the action of RISC requires the endonucleolytic slicer activity of Argonaute2 (Ago2) directed to RNA targets whose sequences are complementary to RISC-incorporated small RNA. To identify RISC components in human cells, we developed an affinity-purification strategy to isolate siRNA-programmed RISC. Here we report the identification of RNA helicase A (RHA) as a human RISC-associated factor. We show that RHA interacts in human cells with siRNA, Ago2, TRBP, and Dicer and functions in the RNAi pathway. In RHA-depleted cells, RNAi was reduced as a consequence of decreased intracellular concentration of active RISC assembled with the guide-strand RNA and Ago2. Our results identify RHA as a RISC component and demonstrate that RHA functions in RISC as an siRNA-loading factor.}, address = {Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA.}, author = {Robb, G Brett B. and Rana, Tariq M M. }, citeulike-article-id = {1343812}, doi = {10.1016/j.molcel.2007.04.016}, issn = {1097-2765}, journal = {Mol Cell}, keywords = {rnai}, month = {May}, number = {4}, pages = {523--537}, posted-at = {2007-05-30 18:57:02}, priority = {2}, title = {RNA Helicase A Interacts with RISC in Human Cells and Functions in RISC Loading.}, url = {http://dx.doi.org/10.1016/j.molcel.2007.04.016}, volume = {26}, year = {2007} }

TY - JOUR ID - citeulike:1343812 L3 - citeulike-article-id:1343812 TI - RNA Helicase A Interacts with RISC in Human Cells and Functions in RISC Loading. JF - Mol Cell VL - 26 IS - 4 SP - 523 EP - 537 AD - Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, MA 01605, USA. SN - 1097-2765 N2 - RNA interference is a conserved pathway of sequence-specific gene silencing that depends on small guide RNAs and the action of proteins assembled in the RNA-induced silencing complex (RISC). Minimally, the action of RISC requires the endonucleolytic slicer activity of Argonaute2 (Ago2) directed to RNA targets whose sequences are complementary to RISC-incorporated small RNA. To identify RISC components in human cells, we developed an affinity-purification strategy to isolate siRNA-programmed RISC. Here we report the identification of RNA helicase A (RHA) as a human RISC-associated factor. We show that RHA interacts in human cells with siRNA, Ago2, TRBP, and Dicer and functions in the RNAi pathway. In RHA-depleted cells, RNAi was reduced as a consequence of decreased intracellular concentration of active RISC assembled with the guide-strand RNA and Ago2. Our results identify RHA as a RISC component and demonstrate that RHA functions in RISC as an siRNA-loading factor. KW - rnai AU - Robb, G Brett AU - Rana, Tariq M PY - 2007/05/25/ UR - http://dx.doi.org/10.1016/j.molcel.2007.04.016 ER -