A genomic approach for the identification and classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae.

@article{citeulike:3023362, abstract = {Using a hierarchical approach, 620 non-essential single-gene yeast deletants generated by EUROFAN I were systematically screened for cell-wall-related phenotypes. By analyzing for altered sensitivity to the presence of Calcofluor white or SDS in the growth medium, altered sensitivity to sonication, or abnormal morphology, 145 (23\%) mutants showing at least one cell wall-related phenotype were selected. These were screened further to identify genes potentially involved in either the biosynthesis, remodeling or coupling of cell wall macromolecules or genes involved in the overall regulation of cell wall construction and to eliminate those genes with a more general, pleiotropic effect. Ninety percent of the mutants selected from the primary tests showed additional cell wall-related phenotypes. When extrapolated to the entire yeast genome, these data indicate that over 1200 genes may directly or indirectly affect cell wall formation and its regulation. Twenty-one mutants with altered levels of beta1,3-glucan synthase activity and five Calcofluor white-resistant mutants with altered levels of chitin synthase activities were found, indicating that the corresponding genes affect beta1,3-glucan or chitin synthesis. By selecting for increased levels of specific cell wall components in the growth medium, we identified 13 genes that are possibly implicated in different steps of cell wall assembly. Furthermore, 14 mutants showed a constitutive activation of the cell wall integrity pathway, suggesting that they participate in the modulation of the pathway either directly acting as signaling components or by triggering the Slt2-dependent compensatory mechanism. In conclusion, our screening approach represents a comprehensive functional analysis on a genomic scale of gene products involved in various aspects of fungal cell wall formation.}, address = {Swammerdam Institute for Life Sciences University of Amsterdam Nieuwe Achtergracht 166 Amsterdam WV 1018 The Netherlands.}, author = {de Groot, P. W. and Ruiz, C. and V\'{a}zquez de Aldana, C. R. and Duenas, E. and Cid, V. J. and Del Rey, F. and Rodr\'{i}quez-Pe\~{n}a, J. M. and P\'{e}rez, P. and Andel, A. and Caub\'{i}n, J. and Arroyo, J. and Garc\'{i}a, J. C. and Gil, C. and Molina, M. and Garc\'{i}a, L. J. and Nombela, C. and Klis, F. M. }, citeulike-article-id = {3023362}, doi = {http://dx.doi.org/10.1002/cfg.85}, issn = {1531-6912}, journal = {Comparative and functional genomics}, keywords = {cell\_wall, fungal, saccharomyces}, number = {3}, pages = {124--142}, posted-at = {2008-07-21 04:08:16}, priority = {2}, title = {A genomic approach for the identification and classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae.}, url = {http://dx.doi.org/10.1002/cfg.85}, volume = {2}, year = {2001} }

TY - JOUR ID - citeulike:3023362 L3 - citeulike-article-id:3023362 TI - A genomic approach for the identification and classification of genes involved in cell wall formation and its regulation in Saccharomyces cerevisiae. JF - Comparative and functional genomics VL - 2 IS - 3 SP - 124 EP - 142 AD - Swammerdam Institute for Life Sciences University of Amsterdam Nieuwe Achtergracht 166 Amsterdam WV 1018 The Netherlands. SN - 1531-6912 N2 - Using a hierarchical approach, 620 non-essential single-gene yeast deletants generated by EUROFAN I were systematically screened for cell-wall-related phenotypes. By analyzing for altered sensitivity to the presence of Calcofluor white or SDS in the growth medium, altered sensitivity to sonication, or abnormal morphology, 145 (23%) mutants showing at least one cell wall-related phenotype were selected. These were screened further to identify genes potentially involved in either the biosynthesis, remodeling or coupling of cell wall macromolecules or genes involved in the overall regulation of cell wall construction and to eliminate those genes with a more general, pleiotropic effect. Ninety percent of the mutants selected from the primary tests showed additional cell wall-related phenotypes. When extrapolated to the entire yeast genome, these data indicate that over 1200 genes may directly or indirectly affect cell wall formation and its regulation. Twenty-one mutants with altered levels of beta1,3-glucan synthase activity and five Calcofluor white-resistant mutants with altered levels of chitin synthase activities were found, indicating that the corresponding genes affect beta1,3-glucan or chitin synthesis. By selecting for increased levels of specific cell wall components in the growth medium, we identified 13 genes that are possibly implicated in different steps of cell wall assembly. Furthermore, 14 mutants showed a constitutive activation of the cell wall integrity pathway, suggesting that they participate in the modulation of the pathway either directly acting as signaling components or by triggering the Slt2-dependent compensatory mechanism. In conclusion, our screening approach represents a comprehensive functional analysis on a genomic scale of gene products involved in various aspects of fungal cell wall formation. KW - cell_wall KW - fungal KW - saccharomyces AU - de Groot, PW AU - Ruiz, C AU - Vázquez de Aldana, CR AU - Duenas, E AU - Cid, VJ AU - Del Rey, F AU - Rodríquez-Peña, JM AU - Pérez, P AU - Andel, A AU - Caubín, J AU - Arroyo, J AU - García, JC AU - Gil, C AU - Molina, M AU - García, LJ AU - Nombela, C AU - Klis, FM PY - 2001/// UR - http://dx.doi.org/10.1002/cfg.85 ER -