A system for functional analysis of Ebola virus glycoprotein.

@article{citeulike:2785899, abstract = {Ebola virus causes hemorrhagic fever in humans and nonhuman primates, resulting in mortality rates of up to 90\%. Studies of this virus have been hampered by its extraordinary pathogenicity, which requires biosafety level 4 containment. To circumvent this problem, we developed a novel complementation system for functional analysis of Ebola virus glycoproteins. It relies on a recombinant vesicular stomatitis virus (VSV) that contains the green fluorescent protein gene instead of the receptor-binding G protein gene (VSVDeltaG*). Herein we show that Ebola Reston virus glycoprotein (ResGP) is efficiently incorporated into VSV particles. This recombinant VSV with integrated ResGP (VSVDeltaG*-ResGP) infected primate cells more efficiently than any of the other mammalian or avian cells examined, in a manner consistent with the host range tropism of Ebola virus, whereas VSVDeltaG* complemented with VSV G protein (VSVDeltaG*-G) efficiently infected the majority of the cells tested. We also tested the utility of this system for investigating the cellular receptors for Ebola virus. Chemical modification of cells to alter their surface proteins markedly reduced their susceptibility to VSVDeltaG*-ResGP but not to VSVDeltaG*-G. These findings suggest that cell surface glycoproteins with N-linked oligosaccharide chains contribute to the entry of Ebola viruses, presumably acting as a specific receptor and/or cofactor for virus entry. Thus, our VSV system should be useful for investigating the functions of glycoproteins from highly pathogenic viruses or those incapable of being cultured in vitro.}, address = {Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 North Lauderdale, P.O. Box 318, Memphis, TN 38101, USA.}, author = {Takada, A. and Robison, C. and Goto, H. and Sanchez, A. and Murti, K. G. and Whitt, M. A. and Kawaoka, Y. }, citeulike-article-id = {2785899}, issn = {0027-8424}, journal = {Proceedings of the National Academy of Sciences of the United States of America}, month = {December}, number = {26}, pages = {14764--14769}, posted-at = {2008-05-11 23:23:34}, priority = {0}, title = {A system for functional analysis of Ebola virus glycoprotein.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/9405687}, volume = {94}, year = {1997} }

TY - JOUR ID - citeulike:2785899 L3 - citeulike-article-id:2785899 TI - A system for functional analysis of Ebola virus glycoprotein. JF - Proceedings of the National Academy of Sciences of the United States of America VL - 94 IS - 26 SP - 14764 EP - 14769 AD - Department of Virology and Molecular Biology, St. Jude Children's Research Hospital, 332 North Lauderdale, P.O. Box 318, Memphis, TN 38101, USA. SN - 0027-8424 N2 - Ebola virus causes hemorrhagic fever in humans and nonhuman primates, resulting in mortality rates of up to 90%. Studies of this virus have been hampered by its extraordinary pathogenicity, which requires biosafety level 4 containment. To circumvent this problem, we developed a novel complementation system for functional analysis of Ebola virus glycoproteins. It relies on a recombinant vesicular stomatitis virus (VSV) that contains the green fluorescent protein gene instead of the receptor-binding G protein gene (VSVDeltaG*). Herein we show that Ebola Reston virus glycoprotein (ResGP) is efficiently incorporated into VSV particles. This recombinant VSV with integrated ResGP (VSVDeltaG*-ResGP) infected primate cells more efficiently than any of the other mammalian or avian cells examined, in a manner consistent with the host range tropism of Ebola virus, whereas VSVDeltaG* complemented with VSV G protein (VSVDeltaG*-G) efficiently infected the majority of the cells tested. We also tested the utility of this system for investigating the cellular receptors for Ebola virus. Chemical modification of cells to alter their surface proteins markedly reduced their susceptibility to VSVDeltaG*-ResGP but not to VSVDeltaG*-G. These findings suggest that cell surface glycoproteins with N-linked oligosaccharide chains contribute to the entry of Ebola viruses, presumably acting as a specific receptor and/or cofactor for virus entry. Thus, our VSV system should be useful for investigating the functions of glycoproteins from highly pathogenic viruses or those incapable of being cultured in vitro. AU - Takada, A AU - Robison, C AU - Goto, H AU - Sanchez, A AU - Murti, KG AU - Whitt, MA AU - Kawaoka, Y PY - 1997/12/23/ UR - http://view.ncbi.nlm.nih.gov/pubmed/9405687 ER -