Identification of proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit

@article{citeulike:2751004, abstract = {Antisera against a synthetic tetradecameric peptide with the sequence DLIQEGNIGLMKAV, which is present in region 2.2 of both [sigma]70 and [sigma]32 subunits of RNA polymerase, cross-reacted with more than 10 proteins including these two [sigma] subunits. Four major species of these cross-reacting proteins (SCRPs) were purified. N-Terminal amino acid sequence analysis revealed that one of them (SCRP-27A) was an as yet unidentified protein while the other three (SCRP-34, SCRP-27B and SCRP-23) were thioredoxin reductase, ribosomal protein S2, and alkyl hydroperoxide reductase, respectively. Immunological competition experiments with various fragments of this [sigma] region 2.2 peptide indicated that the anti-[sigma] peptide serum contained at least three different species of antibodies. All the four SCRPs analyzed here reacted with an antibody against a C-terminus-proximal epitope.}, author = {Ueshima, Rei and Fujita, Nobuyuki and Ishihama, Akira }, citeulike-article-id = {2751004}, doi = {http://dx.doi.org/10.1016/0006-291X(92)90636-Y}, journal = {Biochemical and Biophysical Research Communications}, keywords = {antibodies, cross-reacting, region\_22, sigma}, month = {April}, number = {2}, pages = {634--639}, posted-at = {2008-05-03 23:41:00}, priority = {3}, title = {Identification of proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit}, url = {http://dx.doi.org/10.1016/0006-291X(92)90636-Y}, volume = {184}, year = {1992} }

TY - JOUR ID - citeulike:2751004 L3 - citeulike-article-id:2751004 TI - Identification of proteins cross-reacting with antibodies against region 2.2 peptide of RNA polymerase sigma subunit JF - Biochemical and Biophysical Research Communications VL - 184 IS - 2 SP - 634 EP - 639 N2 - Antisera against a synthetic tetradecameric peptide with the sequence DLIQEGNIGLMKAV, which is present in region 2.2 of both [sigma]70 and [sigma]32 subunits of RNA polymerase, cross-reacted with more than 10 proteins including these two [sigma] subunits. Four major species of these cross-reacting proteins (SCRPs) were purified. N-Terminal amino acid sequence analysis revealed that one of them (SCRP-27A) was an as yet unidentified protein while the other three (SCRP-34, SCRP-27B and SCRP-23) were thioredoxin reductase, ribosomal protein S2, and alkyl hydroperoxide reductase, respectively. Immunological competition experiments with various fragments of this [sigma] region 2.2 peptide indicated that the anti-[sigma] peptide serum contained at least three different species of antibodies. All the four SCRPs analyzed here reacted with an antibody against a C-terminus-proximal epitope. KW - antibodies KW - cross-reacting KW - region_22 KW - sigma AU - Ueshima, Rei AU - Fujita, Nobuyuki AU - Ishihama, Akira PY - 1992/04/30/ UR - http://dx.doi.org/10.1016/0006-291X(92)90636-Y ER -