A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids.

@article{citeulike:808774, abstract = {We show that brome mosaic virus (BMV) RNA replication protein 1a, 2a polymerase, and a cis-acting replication signal recapitulate the functions of Gag, Pol, and RNA packaging signals in conventional retrovirus and foamy virus cores. Prior to RNA replication, 1a forms spherules budding into the endoplasmic reticulum membrane, sequestering viral positive-strand RNA templates in a nuclease-resistant, detergent-susceptible state. When expressed, 2a polymerase colocalizes in these spherules, which become the sites of viral RNA synthesis and retain negative-strand templates for positive-strand RNA synthesis. These results explain many features of replication by numerous positive strand RNA viruses and reveal that these viruses, reverse transcribing viruses, and dsRNA viruses share fundamental similarities in replication and may have common evolutionary origins.}, address = {Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, Madison, WI 53706, USA.}, author = {Schwartz, M. and Chen, J. and Janda, M. and Sullivan, M. and den Boon, J. and Ahlquist, P. }, citeulike-article-id = {808774}, issn = {1097-2765}, journal = {Mol Cell}, keywords = {bromo, replication}, month = {March}, number = {3}, pages = {505--514}, posted-at = {2006-08-21 00:14:24}, priority = {0}, title = {A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/11931759}, volume = {9}, year = {2002} }

TY - JOUR ID - citeulike:808774 L3 - citeulike-article-id:808774 TI - A positive-strand RNA virus replication complex parallels form and function of retrovirus capsids. JF - Mol Cell VL - 9 IS - 3 SP - 505 EP - 514 AD - Institute for Molecular Virology and Howard Hughes Medical Institute, University of Wisconsin, Madison, Madison, WI 53706, USA. SN - 1097-2765 N2 - We show that brome mosaic virus (BMV) RNA replication protein 1a, 2a polymerase, and a cis-acting replication signal recapitulate the functions of Gag, Pol, and RNA packaging signals in conventional retrovirus and foamy virus cores. Prior to RNA replication, 1a forms spherules budding into the endoplasmic reticulum membrane, sequestering viral positive-strand RNA templates in a nuclease-resistant, detergent-susceptible state. When expressed, 2a polymerase colocalizes in these spherules, which become the sites of viral RNA synthesis and retain negative-strand templates for positive-strand RNA synthesis. These results explain many features of replication by numerous positive strand RNA viruses and reveal that these viruses, reverse transcribing viruses, and dsRNA viruses share fundamental similarities in replication and may have common evolutionary origins. KW - bromo KW - replication AU - Schwartz, M AU - Chen, J AU - Janda, M AU - Sullivan, M AU - den Boon, J AU - Ahlquist, P PY - 2002/03// UR - http://view.ncbi.nlm.nih.gov/pubmed/11931759 ER -