Pericentromeric regions of soybean (Glycine max L. Merr.) chromosomes consist of retroelements and tandemly repeated DNA and are structurally and evolutionarily labile.

@article{citeulike:2743245, abstract = {Little is known about the physical makeup of heterochromatin in the soybean (Glycine max L. Merr.) genome. Using DNA sequencing and molecular cytogenetics, an initial analysis of the repetitive fraction of the soybean genome is presented. BAC 076J21, derived from linkage group L, has sequences conserved in the pericentromeric heterochromatin of all 20 chromosomes. FISH analysis of this BAC and three subclones on pachytene chromosomes revealed relatively strict partitioning of the heterochromatic and euchromatic regions. Sequence analysis showed that this BAC consists primarily of repetitive sequences such as a 102-bp tandem repeat with sequence identity to a previously characterized approximately 120-bp repeat (STR120). Fragments of Calypso-like retroelements, a recently inserted SIRE1 element, and a SIRE1 solo LTR were present within this BAC. Some of these sequences are methylated and are not conserved outside of G. max and G. soja, a close relative of soybean, except for STR102, which hybridized to a restriction fragment from G. latifolia. These data present a picture of the repetitive fraction of the soybean genome that is highly concentrated in the pericentromeric regions, consisting of rapidly evolving tandem repeats with interspersed retroelements.}, address = {Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA.}, author = {Lin, J. Y. and Jacobus, B. H. and SanMiguel, P. and Walling, J. G. and Yuan, Y. and Shoemaker, R. C. and Young, N. D. and Jackson, S. A. }, citeulike-article-id = {2743245}, doi = {10.1534/genetics.105.041616}, issn = {0016-6731}, journal = {Genetics}, keywords = {genomics, sinteny, soybean}, month = {July}, number = {3}, pages = {1221--1230}, posted-at = {2008-05-01 19:43:43}, priority = {0}, title = {Pericentromeric regions of soybean (Glycine max L. Merr.) chromosomes consist of retroelements and tandemly repeated DNA and are structurally and evolutionarily labile.}, url = {http://dx.doi.org/10.1534/genetics.105.041616}, volume = {170}, year = {2005} }

TY - JOUR ID - citeulike:2743245 L3 - citeulike-article-id:2743245 TI - Pericentromeric regions of soybean (Glycine max L. Merr.) chromosomes consist of retroelements and tandemly repeated DNA and are structurally and evolutionarily labile. JF - Genetics VL - 170 IS - 3 SP - 1221 EP - 1230 AD - Department of Agronomy, Purdue University, West Lafayette, Indiana 47907, USA. SN - 0016-6731 N2 - Little is known about the physical makeup of heterochromatin in the soybean (Glycine max L. Merr.) genome. Using DNA sequencing and molecular cytogenetics, an initial analysis of the repetitive fraction of the soybean genome is presented. BAC 076J21, derived from linkage group L, has sequences conserved in the pericentromeric heterochromatin of all 20 chromosomes. FISH analysis of this BAC and three subclones on pachytene chromosomes revealed relatively strict partitioning of the heterochromatic and euchromatic regions. Sequence analysis showed that this BAC consists primarily of repetitive sequences such as a 102-bp tandem repeat with sequence identity to a previously characterized approximately 120-bp repeat (STR120). Fragments of Calypso-like retroelements, a recently inserted SIRE1 element, and a SIRE1 solo LTR were present within this BAC. Some of these sequences are methylated and are not conserved outside of G. max and G. soja, a close relative of soybean, except for STR102, which hybridized to a restriction fragment from G. latifolia. These data present a picture of the repetitive fraction of the soybean genome that is highly concentrated in the pericentromeric regions, consisting of rapidly evolving tandem repeats with interspersed retroelements. KW - genomics KW - sinteny KW - soybean AU - Lin, JY AU - Jacobus, BH AU - SanMiguel, P AU - Walling, JG AU - Yuan, Y AU - Shoemaker, RC AU - Young, ND AU - Jackson, SA PY - 2005/07// UR - http://dx.doi.org/10.1534/genetics.105.041616 ER -