Generation of Gradients Having Complex Shapes Using Microfluidic Networks

@article{dertinger01, abstract = {Abstract: This paper describes the generation of gradients having complex shapes in solution using microfluidic networks. Flowing multiple streams of fluid each carrying different concentrations of substances laminarly and side-by-side generated step concentration gradients perpendicular to the direction of the flow. Appropriately designed networks of microchannels for controlled diffusive mixing of substances generated a range of shapes for the gradients, including linear, parabolic, and periodic. The lateral dimensions of the gradients ranged from 900 to 2200 m. This paper also demonstrates the generation of overlapping gradients composed of different species. Since solutions in the microfluidic network exist as steady states and are continuously renewed, the gradients established in the capillaries are spatially and temporally constant and can be maintained easily for periods of hours. Using laminar flow to generate gradients should be useful in both biological and nonbiological research.}, address = {Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138}, author = {Dertinger, S. K. W. and Chiu, D. T. and Jeon, N. L. and Whitesides, G. M. }, citeulike-article-id = {2975613}, doi = {http://dx.doi.org/10.1021/ac001132d}, journal = {Anal. Chem.}, keywords = {microfluidics}, month = {March}, number = {6}, pages = {1240--1246}, posted-at = {2008-07-09 11:24:55}, priority = {2}, title = {Generation of Gradients Having Complex Shapes Using Microfluidic Networks}, url = {http://dx.doi.org/10.1021/ac001132d}, volume = {73}, year = {2001} }

TY - JOUR ID - dertinger01 L3 - citeulike-article-id:2975613 TI - Generation of Gradients Having Complex Shapes Using Microfluidic Networks JF - Anal. Chem. VL - 73 IS - 6 SP - 1240 EP - 1246 AD - Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford Street, Cambridge, Massachusetts 02138 N2 - Abstract: This paper describes the generation of gradients having complex shapes in solution using microfluidic networks. Flowing multiple streams of fluid each carrying different concentrations of substances laminarly and side-by-side generated step concentration gradients perpendicular to the direction of the flow. Appropriately designed networks of microchannels for controlled diffusive mixing of substances generated a range of shapes for the gradients, including linear, parabolic, and periodic. The lateral dimensions of the gradients ranged from 900 to 2200 m. This paper also demonstrates the generation of overlapping gradients composed of different species. Since solutions in the microfluidic network exist as steady states and are continuously renewed, the gradients established in the capillaries are spatially and temporally constant and can be maintained easily for periods of hours. Using laminar flow to generate gradients should be useful in both biological and nonbiological research. KW - microfluidics AU - Dertinger, SKW AU - Chiu, DT AU - Jeon, NL AU - Whitesides, GM PY - 2001/03/15/ UR - http://dx.doi.org/10.1021/ac001132d ER -