The untiring search for the most complete proteome representation: reviewing the methods

@article{citeulike:2847164, abstract = {Proteomic research has proved valuable for understanding the molecular mechanisms of biological processes, as well as in the search for biomarkers for a variety of diseases which lack a molecular diagnostic. While several new approaches are being developed, two-dimensional (2-DE) gel electrophoresis is still one of the most commonly used techniques, despite its many limitations. However, for biomarker research, 2-DE gel electrophoresis alone does not fulfill the necessary pre-requisites. If such a technique is utilized exclusively, a great part of a given proteome remains unseen. Therefore, very precise and sensitive techniques are needed. Here, we present a brief review of known methodologies that try to overcome the limitations of conventional proteome analysis as well as their respective advantages and limitations. 10.1093/bfgp/eln023}, author = {de Souza, Daniel M. and Oliveira, Bruno M. and Castro-Dias, Erich and Winck, Flavia V. and Horiuchi, Ricardo S. and Baldasso, Paulo A. and Caetano, Hugo T. and Pires, Natalia K. and Marangoni, Sergio and Novello, Jose C. }, citeulike-article-id = {2847164}, doi = {10.1093/bfgp/eln023}, journal = {Brief Funct Genomic Proteomic}, keywords = {methods, proteome}, month = {May}, pages = {eln023+}, posted-at = {2008-05-30 13:02:20}, priority = {2}, title = {The untiring search for the most complete proteome representation: reviewing the methods}, url = {http://dx.doi.org/10.1093/bfgp/eln023}, year = {2008} }

TY - JOUR ID - citeulike:2847164 L3 - citeulike-article-id:2847164 TI - The untiring search for the most complete proteome representation: reviewing the methods JF - Brief Funct Genomic Proteomic SP - eln023 N2 - Proteomic research has proved valuable for understanding the molecular mechanisms of biological processes, as well as in the search for biomarkers for a variety of diseases which lack a molecular diagnostic. While several new approaches are being developed, two-dimensional (2-DE) gel electrophoresis is still one of the most commonly used techniques, despite its many limitations. However, for biomarker research, 2-DE gel electrophoresis alone does not fulfill the necessary pre-requisites. If such a technique is utilized exclusively, a great part of a given proteome remains unseen. Therefore, very precise and sensitive techniques are needed. Here, we present a brief review of known methodologies that try to overcome the limitations of conventional proteome analysis as well as their respective advantages and limitations. 10.1093/bfgp/eln023 KW - methods KW - proteome AU - de Souza, Daniel AU - Oliveira, Bruno AU - Castro-Dias, Erich AU - Winck, Flavia AU - Horiuchi, Ricardo AU - Baldasso, Paulo AU - Caetano, Hugo AU - Pires, Natalia AU - Marangoni, Sergio AU - Novello, Jose PY - 2008/05/29/ UR - http://dx.doi.org/10.1093/bfgp/eln023 ER -