Cholinesterase measurements with an automated kit.

@article{citeulike:2711128, abstract = {BACKGROUND: The Test-mate kit determines acetylcholinesterase (AChE, EC 3.1.1.7) and hemoglobin content of a drop of blood, displaying enzyme activities normalized to 25 degrees C. Previous models produced inconsistent results at different temperatures. This report focuses on the current model, ChE 400, and two instruments of a previous OP model. METHODS: AChE activities were determined by the Ellman assay, using the three kits and a 96-well microplate reader. Temperatures ranged from 10 to 37 degrees C. Fetal bovine serum was the source of AChE. RESULTS: Normalized activities decreased below 20 degrees C in the ChE model and below 25 degrees C in the OP models. Activities of the same serum sample differed between the three Test-mate kits, ranging from 1.03 to 1.49 micromoles/min/ml. Percent errors were greater than with the microplate reader at all temperatures. CONCLUSIONS: Neither we nor the manufacturer recommend the current Test-mate model for fieldwork. Nevertheless, there have been field measurements with Test-Mate kits, and we recommend that an enzyme activity standard be run in parallel with their use.}, address = {Department of Animal Science, University of California, Davis, California 95616, USA.}, author = {Oliveira, G. H. and Henderson, J. D. and Wilson, B. W. }, citeulike-article-id = {2711128}, doi = {10.1002/ajim.10105}, issn = {0271-3586}, journal = {American journal of industrial medicine}, keywords = {pesticides}, month = {August}, pages = {49--53}, posted-at = {2008-04-24 02:05:21}, priority = {2}, title = {Cholinesterase measurements with an automated kit.}, url = {http://dx.doi.org/10.1002/ajim.10105}, volume = {Suppl 2}, year = {2002} }

TY - JOUR ID - citeulike:2711128 L3 - citeulike-article-id:2711128 TI - Cholinesterase measurements with an automated kit. JF - American journal of industrial medicine VL - Suppl 2 SP - 49 EP - 53 AD - Department of Animal Science, University of California, Davis, California 95616, USA. SN - 0271-3586 N2 - BACKGROUND: The Test-mate kit determines acetylcholinesterase (AChE, EC 3.1.1.7) and hemoglobin content of a drop of blood, displaying enzyme activities normalized to 25 degrees C. Previous models produced inconsistent results at different temperatures. This report focuses on the current model, ChE 400, and two instruments of a previous OP model. METHODS: AChE activities were determined by the Ellman assay, using the three kits and a 96-well microplate reader. Temperatures ranged from 10 to 37 degrees C. Fetal bovine serum was the source of AChE. RESULTS: Normalized activities decreased below 20 degrees C in the ChE model and below 25 degrees C in the OP models. Activities of the same serum sample differed between the three Test-mate kits, ranging from 1.03 to 1.49 micromoles/min/ml. Percent errors were greater than with the microplate reader at all temperatures. CONCLUSIONS: Neither we nor the manufacturer recommend the current Test-mate model for fieldwork. Nevertheless, there have been field measurements with Test-Mate kits, and we recommend that an enzyme activity standard be run in parallel with their use. KW - pesticides AU - Oliveira, GH AU - Henderson, JD AU - Wilson, BW PY - 2002/08// UR - http://dx.doi.org/10.1002/ajim.10105 ER -