Transgenic Inhibition of Synaptic Transmission Reveals Role of CA3 Output in Hippocampal Learning

by: Toshiaki Nakashiba, Jennie Z Young, Thomas J Mchugh, Derek L Buhl, Susumu Tonegawa

Science (24 January 2008), 1151120.

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Abstract

The hippocampus is an area of the brain involved in learning and memory. It contains parallel excitatory pathways referred to as the trisynaptic pathway (which carries information from the entorhinal cortex [->] dentate gyrus [->] CA3 [->] CA1 [->] entorhinal cortex) and the monosynaptic pathway (which connects entorhinal cortex [->] CA1 [->] entorhinal cortex). We developed a generally applicable tetanus toxin-based method for transgenic mice that permits inducible and reversible inhibition of synaptic transmission and applied it to the trisynaptic pathway while preserving transmission in the monosynaptic pathway. We found that synaptic output from CA3 in the trisynaptic pathway is dispensable and the short monosynaptic pathway is sufficient for incremental spatial learning. In contrast, the full trisynaptic pathway containing CA3 is required for rapid, one-trial contextual learning, for pattern completionbased memory recall and for spatial tuning of CA1 cells. 10.1126/science.1151120

@article{citeulike:2286335, abstract = {The hippocampus is an area of the brain involved in learning and memory. It contains parallel excitatory pathways referred to as the trisynaptic pathway (which carries information from the entorhinal cortex [-\>] dentate gyrus [-\>] CA3 [-\>] CA1 [-\>] entorhinal cortex) and the monosynaptic pathway (which connects entorhinal cortex [-\>] CA1 [-\>] entorhinal cortex). We developed a generally applicable tetanus toxin-based method for transgenic mice that permits inducible and reversible inhibition of synaptic transmission and applied it to the trisynaptic pathway while preserving transmission in the monosynaptic pathway. We found that synaptic output from CA3 in the trisynaptic pathway is dispensable and the short monosynaptic pathway is sufficient for incremental spatial learning. In contrast, the full trisynaptic pathway containing CA3 is required for rapid, one-trial contextual learning, for pattern completionbased memory recall and for spatial tuning of CA1 cells. 10.1126/science.1151120}, author = {Nakashiba, Toshiaki and Young, Jennie Z. and Mchugh, Thomas J. and Buhl, Derek L. and Tonegawa, Susumu }, citeulike-article-id = {2286335}, doi = {10.1126/science.1151120}, journal = {Science}, keywords = {ca3, gene\_silencing, hippocampus, methods}, month = {January}, pages = {1151120+}, posted-at = {2008-02-29 10:18:39}, priority = {2}, title = {Transgenic Inhibition of Synaptic Transmission Reveals Role of CA3 Output in Hippocampal Learning}, url = {http://dx.doi.org/10.1126/science.1151120}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2286335 L3 - citeulike-article-id:2286335 TI - Transgenic Inhibition of Synaptic Transmission Reveals Role of CA3 Output in Hippocampal Learning JF - Science SP - 1151120 N2 - The hippocampus is an area of the brain involved in learning and memory. It contains parallel excitatory pathways referred to as the trisynaptic pathway (which carries information from the entorhinal cortex [->] dentate gyrus [->] CA3 [->] CA1 [->] entorhinal cortex) and the monosynaptic pathway (which connects entorhinal cortex [->] CA1 [->] entorhinal cortex). We developed a generally applicable tetanus toxin-based method for transgenic mice that permits inducible and reversible inhibition of synaptic transmission and applied it to the trisynaptic pathway while preserving transmission in the monosynaptic pathway. We found that synaptic output from CA3 in the trisynaptic pathway is dispensable and the short monosynaptic pathway is sufficient for incremental spatial learning. In contrast, the full trisynaptic pathway containing CA3 is required for rapid, one-trial contextual learning, for pattern completionbased memory recall and for spatial tuning of CA1 cells. 10.1126/science.1151120 KW - ca3 KW - gene_silencing KW - hippocampus KW - methods AU - Nakashiba, Toshiaki AU - Young, Jennie AU - Mchugh, Thomas AU - Buhl, Derek AU - Tonegawa, Susumu PY - 2008/01/24/ UR - http://dx.doi.org/10.1126/science.1151120 ER -

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