Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF

@article{citeulike:2974032, abstract = {BACKGROUND:Enterococcus faecalis has emerged as a major hospital pathogen. To explore its diversity, we sequenced E. faecalis strain OG1RF that is commonly used for molecular manipulation and virulence studies. RESULTS:The 2,739,625 base pair chromosome of OG1RF was found to contain ~232 kilobases unique to this strain compared to V583, the only publically available sequenced strain. Almost no mobile genetic elements were found in OG1RF. The 64 areas of divergence were classified into three categories. First, OG1RF carries 39 unique regions, including two CRISPR loci and a new WxL locus. Second, we found 9 replacements where a sequence specific to V583 was substituted by a sequence specific to OG1RF. For example, the iol operon of OG1RF replaces a possible prophage and the vanB transposon in V583. Finally, we found 16 regions that were present in V583 but missing from OG1RF including the proposed pathogenicity island, several probable prophages, and the cpsCDEFGHIJK capsular polysaccharide operon. OG1RF was more rapidly but less frequently lethal than V583 in the mouse peritonitis model and considerably outcompeted V583 in a murine model of urinary tract infections.CONCLUSION:In conclusion, E. faecalis OG1RF carries a number of unique loci compared to V583, but the almost complete lack of mobile genetic elements demonstrates that this is not a defining feature of the species. Additionally, OG1RF's effect in experimental models suggests that mediators of virulence may be diverse between different E. faecalis strains and that virulence is not dependent of the presence of mobile genetic elements.}, author = {Bourgogne, Agathe and Garsin, Danielle and Qin, Xiang and Singh, Kavindra and Sillanpaa, Jouko and Yerrapragada, Shailaja and Ding, Yan and Rocha, Shannon D. and Buhay, Christian and Shen, Hua and Chen, Guan and Williams, Gabrielle and Muzny, Donna and Maadani, Arash and Fox, Kristina and Gioia, Jason and Chen, Lei and Shang, Yue and Arias, Cesar and Nallapareddy, Sreedhar and Zhao, Meng and Prakash, Vittal and Chowdhury, Shahreen and Jiang, Huaiyang and Gibbs, Richard and Murray, Barbara and Highlander, Sarah and Weinstock, George }, citeulike-article-id = {2974032}, doi = {10.1186/gb-2008-9-7-r110}, journal = {Genome Biology}, keywords = {bacteria, enterococcus, genomics, pathogen, virulence}, number = {7}, posted-at = {2008-07-09 03:42:20}, priority = {2}, title = {Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF}, url = {http://dx.doi.org/10.1186/gb-2008-9-7-r110}, volume = {9}, year = {2008} }

TY - JOUR ID - citeulike:2974032 L3 - citeulike-article-id:2974032 TI - Large scale variation in Enterococcus faecalis illustrated by the genome analysis of strain OG1RF JF - Genome Biology VL - 9 IS - 7 N2 - BACKGROUND:Enterococcus faecalis has emerged as a major hospital pathogen. To explore its diversity, we sequenced E. faecalis strain OG1RF that is commonly used for molecular manipulation and virulence studies. RESULTS:The 2,739,625 base pair chromosome of OG1RF was found to contain ~232 kilobases unique to this strain compared to V583, the only publically available sequenced strain. Almost no mobile genetic elements were found in OG1RF. The 64 areas of divergence were classified into three categories. First, OG1RF carries 39 unique regions, including two CRISPR loci and a new WxL locus. Second, we found 9 replacements where a sequence specific to V583 was substituted by a sequence specific to OG1RF. For example, the iol operon of OG1RF replaces a possible prophage and the vanB transposon in V583. Finally, we found 16 regions that were present in V583 but missing from OG1RF including the proposed pathogenicity island, several probable prophages, and the cpsCDEFGHIJK capsular polysaccharide operon. OG1RF was more rapidly but less frequently lethal than V583 in the mouse peritonitis model and considerably outcompeted V583 in a murine model of urinary tract infections.CONCLUSION:In conclusion, E. faecalis OG1RF carries a number of unique loci compared to V583, but the almost complete lack of mobile genetic elements demonstrates that this is not a defining feature of the species. Additionally, OG1RF's effect in experimental models suggests that mediators of virulence may be diverse between different E. faecalis strains and that virulence is not dependent of the presence of mobile genetic elements. KW - bacteria KW - enterococcus KW - genomics KW - pathogen KW - virulence AU - Bourgogne, Agathe AU - Garsin, Danielle AU - Qin, Xiang AU - Singh, Kavindra AU - Sillanpaa, Jouko AU - Yerrapragada, Shailaja AU - Ding, Yan AU - Rocha, Shannon AU - Buhay, Christian AU - Shen, Hua AU - Chen, Guan AU - Williams, Gabrielle AU - Muzny, Donna AU - Maadani, Arash AU - Fox, Kristina AU - Gioia, Jason AU - Chen, Lei AU - Shang, Yue AU - Arias, Cesar AU - Nallapareddy, Sreedhar AU - Zhao, Meng AU - Prakash, Vittal AU - Chowdhury, Shahreen AU - Jiang, Huaiyang AU - Gibbs, Richard AU - Murray, Barbara AU - Highlander, Sarah AU - Weinstock, George PY - 2008/// UR - http://dx.doi.org/10.1186/gb-2008-9-7-r110 ER -