The NosX and NirX proteins of Paracoccus denitrificans are functional homologues: their role in maturation of nitrous oxide reductase.

@article{citeulike:2783983, abstract = {The nos (nitrous oxide reductase) operon of Paracoccus denitrificans contains a nosX gene homologous to those found in the nos operons of other denitrifiers. NosX is also homologous to NirX, which is so far unique to P. denitrificans. Single mutations of these genes did not result in any apparent phenotype, but a double nosX nirX mutant was unable to reduce nitrous oxide. Promoter-lacZ assays and immunoblotting against nitrous oxide reductase showed that the defect was not due to failure of expression of nosZ, the structural gene for nitrous oxide reductase. Electron paramagnetic resonance spectroscopy showed that nitrous oxide reductase in cells of the double mutant lacked the Cu(A) center. A twin-arginine motif in both NosX and NirX suggests that the NosX proteins are exported to the periplasm via the TAT translocon.}, address = {Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, Amsterdam, The Netherlands.}, author = {Saunders, N. F. and Hornberg, J. J. and Reijnders, W. N. and Westerhoff, H. V. and de Vries, S. and van Spanning, R. J. }, citeulike-article-id = {2783983}, issn = {0021-9193}, journal = {Journal of bacteriology}, keywords = {enzyme, maturation, nirx, nitrous, nosx, oxide, paracoccus, reductase}, month = {September}, number = {18}, pages = {5211--5217}, posted-at = {2008-05-11 10:24:14}, priority = {2}, title = {The NosX and NirX proteins of Paracoccus denitrificans are functional homologues: their role in maturation of nitrous oxide reductase.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/10960107}, volume = {182}, year = {2000} }

TY - JOUR ID - citeulike:2783983 TI - The NosX and NirX proteins of Paracoccus denitrificans are functional homologues: their role in maturation of nitrous oxide reductase. JF - Journal of bacteriology VL - 182 IS - 18 SP - 5211 EP - 5217 AD - Department of Molecular Cell Physiology, Faculty of Biology, BioCentrum Amsterdam, Vrije Universiteit, Amsterdam, The Netherlands. SN - 0021-9193 N2 - The nos (nitrous oxide reductase) operon of Paracoccus denitrificans contains a nosX gene homologous to those found in the nos operons of other denitrifiers. NosX is also homologous to NirX, which is so far unique to P. denitrificans. Single mutations of these genes did not result in any apparent phenotype, but a double nosX nirX mutant was unable to reduce nitrous oxide. Promoter-lacZ assays and immunoblotting against nitrous oxide reductase showed that the defect was not due to failure of expression of nosZ, the structural gene for nitrous oxide reductase. Electron paramagnetic resonance spectroscopy showed that nitrous oxide reductase in cells of the double mutant lacked the Cu(A) center. A twin-arginine motif in both NosX and NirX suggests that the NosX proteins are exported to the periplasm via the TAT translocon. KW - enzyme KW - maturation KW - nirx KW - nitrous KW - nosx KW - oxide KW - paracoccus KW - reductase AU - Saunders, NF AU - Hornberg, JJ AU - Reijnders, WN AU - Westerhoff, HV AU - de Vries, S AU - van Spanning, RJ PY - 2000/09// UR - http://view.ncbi.nlm.nih.gov/pubmed/10960107 ER -