Crystal Structure of the FK506 Binding Domain of Plasmodium falciparum FKBP35 in Complex with FK506

@article{citeulike:2774439, abstract = {Abstract: The emergence of multi-drug-resistant strains of Plasmodium parasites has prompted the search for alternative therapeutic strategies for combating malaria. One possible strategy is to exploit existing drugs as lead compounds. FK506 is currently used in the clinic for preventing transplant rejection. It binds to a ±/² protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. We report here the crystallographic structure at 2.35 \r{A} resolution of PfFKBD complexed with FK506. Compared to the human FKBP12FK506 complex reported earlier, the structure reveals structural differences in the ²5²6 segment that lines the FK506 binding site. The presence in PfFKBD of Cys-106 and Ser-109 (substituting for His-87 and Ile-90, respectively, in human FKBP12), which are 45 \r{A} from the nearest atom of the FK506 compound, suggests possible routes for the rational design of analogues of FK506 with specific antiparasitic activity. Upon ligand binding, several conformational changes occur in PfFKBD, including aromatic residues that shape the FK506 binding pocket as shown by NMR studies. A microarray analysis suggests that FK506 and cyclosporine A (CsA) might inhibit parasite development by interfering with the same signaling pathways.}, address = {School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, and AFMB, CNRS UMR6098, Marseille 13288, France}, author = {Kotaka, Masayo and Ye, Hong and Alag, Reema and Hu, Guangan and Bozdech, Zbynek and Preiser, Peter R. and Yoon, Ho S. and Lescar, Julien }, citeulike-article-id = {2774439}, doi = {10.1021/bi800004u}, journal = {Biochemistry}, keywords = {complex, crystallography, fkbp, ligand, plasmodium, structure}, month = {May}, posted-at = {2008-05-09 06:57:49}, priority = {2}, title = {Crystal Structure of the FK506 Binding Domain of Plasmodium falciparum FKBP35 in Complex with FK506}, url = {http://dx.doi.org/10.1021/bi800004u}, year = {2008} }

TY - JOUR ID - citeulike:2774439 L3 - citeulike-article-id:2774439 TI - Crystal Structure of the FK506 Binding Domain of Plasmodium falciparum FKBP35 in Complex with FK506 JF - Biochemistry AD - School of Biological Sciences, Nanyang Technological University, 60 Nanyang Drive, Singapore 637551, and AFMB, CNRS UMR6098, Marseille 13288, France N2 - Abstract: The emergence of multi-drug-resistant strains of Plasmodium parasites has prompted the search for alternative therapeutic strategies for combating malaria. One possible strategy is to exploit existing drugs as lead compounds. FK506 is currently used in the clinic for preventing transplant rejection. It binds to a ±/² protein module of approximately 120 amino acids known as the FK506 binding domain (FKBD), which is found in various organisms, including human, yeast, and Plasmodium falciparum (PfFKBD). Antiparasitic effects of FK506 and its analogues devoid of immunosuppressive activities have been demonstrated. We report here the crystallographic structure at 2.35 Å resolution of PfFKBD complexed with FK506. Compared to the human FKBP12FK506 complex reported earlier, the structure reveals structural differences in the ²5²6 segment that lines the FK506 binding site. The presence in PfFKBD of Cys-106 and Ser-109 (substituting for His-87 and Ile-90, respectively, in human FKBP12), which are 45 Å from the nearest atom of the FK506 compound, suggests possible routes for the rational design of analogues of FK506 with specific antiparasitic activity. Upon ligand binding, several conformational changes occur in PfFKBD, including aromatic residues that shape the FK506 binding pocket as shown by NMR studies. A microarray analysis suggests that FK506 and cyclosporine A (CsA) might inhibit parasite development by interfering with the same signaling pathways. KW - complex KW - crystallography KW - fkbp KW - ligand KW - plasmodium KW - structure AU - Kotaka, Masayo AU - Ye, Hong AU - Alag, Reema AU - Hu, Guangan AU - Bozdech, Zbynek AU - Preiser, Peter AU - Yoon, Ho AU - Lescar, Julien PY - 2008/05/09/ UR - http://dx.doi.org/10.1021/bi800004u ER -