Phosphorylation of Retinoblastoma Protein by Viral Protein with Cyclin-Dependent Kinase Function

@article{citeulike:2773673, abstract = {As obligate intracellular parasites, viruses expertly modify cellular processes to facilitate their replication and spread, often by encoding genes that mimic the functions of cellular proteins while lacking regulatory features that modify their activity. We show that the human cytomegalovirus UL97 protein has activities similar to cellular cyclin-cyclin-dependent kinase (CDK) complexes. UL97 phosphorylated and inactivated the retinoblastoma tumor suppressor, stimulated cell cycle progression in mammalian cells, and rescued proliferation of Saccharomyces cerevisiae lacking CDK activity. UL97 is not inhibited by the CDK inhibitor p21 and lacks amino acid residues conserved in the CDKs that permit the attenuation of kinase activity. Thus, UL97 represents a functional ortholog of cellular CDKs that is immune from normal CDK control mechanisms. 10.1126/science.1152095}, author = {Hume, Adam J. and Finkel, Jonathan S. and Kamil, Jeremy P. and Coen, Donald M. and Culbertson, Michael R. and Kalejta, Robert F. }, citeulike-article-id = {2773673}, doi = {10.1126/science.1152095}, journal = {Science}, keywords = {cyclin-dependent, kinase, phosphorylation, retinoblastoma, virus}, month = {May}, number = {5877}, pages = {797--799}, posted-at = {2008-05-09 00:33:27}, priority = {2}, title = {Phosphorylation of Retinoblastoma Protein by Viral Protein with Cyclin-Dependent Kinase Function}, url = {http://dx.doi.org/10.1126/science.1152095}, volume = {320}, year = {2008} }

TY - JOUR ID - citeulike:2773673 L3 - citeulike-article-id:2773673 TI - Phosphorylation of Retinoblastoma Protein by Viral Protein with Cyclin-Dependent Kinase Function JF - Science VL - 320 IS - 5877 SP - 797 EP - 799 N2 - As obligate intracellular parasites, viruses expertly modify cellular processes to facilitate their replication and spread, often by encoding genes that mimic the functions of cellular proteins while lacking regulatory features that modify their activity. We show that the human cytomegalovirus UL97 protein has activities similar to cellular cyclin-cyclin-dependent kinase (CDK) complexes. UL97 phosphorylated and inactivated the retinoblastoma tumor suppressor, stimulated cell cycle progression in mammalian cells, and rescued proliferation of Saccharomyces cerevisiae lacking CDK activity. UL97 is not inhibited by the CDK inhibitor p21 and lacks amino acid residues conserved in the CDKs that permit the attenuation of kinase activity. Thus, UL97 represents a functional ortholog of cellular CDKs that is immune from normal CDK control mechanisms. 10.1126/science.1152095 KW - cyclin-dependent KW - kinase KW - phosphorylation KW - retinoblastoma KW - virus AU - Hume, Adam AU - Finkel, Jonathan AU - Kamil, Jeremy AU - Coen, Donald AU - Culbertson, Michael AU - Kalejta, Robert PY - 2008/05/09/ UR - http://dx.doi.org/10.1126/science.1152095 ER -