Classical nuclear localization signals: definition, function, and interaction with importin alpha.

@article{citeulike:2404964, abstract = {The best understood system for the transport of macromolecules between the cytoplasm and the nucleus is the classical nuclear import pathway. In this pathway, a protein containing a classical basic nuclear localization signal (NLS) is imported by a heterodimeric import receptor consisting of the beta-karyopherin importin beta, which mediates interactions with the nuclear pore complex, and the adaptor protein importin alpha, which directly binds the classical NLS. Here we review recent studies that have advanced our understanding of this pathway and also take a bioinformatics approach to analyze the likely prevalence of this system in vivo. Finally, we describe how a predicted NLS within a protein of interest can be confirmed experimentally to be functionally important.}, address = {Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia 30322, USA.}, author = {Lange, A. and Mills, R. E. and Lange, C. J. and Stewart, M. and Devine, S. E. and Corbett, A. H. }, citeulike-article-id = {2404964}, doi = {10.1074/jbc.R600026200}, issn = {0021-9258}, journal = {J Biol Chem}, keywords = {bioinformatics, importin, nls, nuclear, review}, month = {February}, number = {8}, pages = {5101--5105}, posted-at = {2008-02-21 01:55:29}, priority = {2}, title = {Classical nuclear localization signals: definition, function, and interaction with importin alpha.}, url = {http://dx.doi.org/10.1074/jbc.R600026200}, volume = {282}, year = {2007} }

TY - JOUR ID - citeulike:2404964 L3 - citeulike-article-id:2404964 TI - Classical nuclear localization signals: definition, function, and interaction with importin alpha. JF - J Biol Chem VL - 282 IS - 8 SP - 5101 EP - 5105 AD - Department of Biochemistry, School of Medicine, Emory University, Atlanta, Georgia 30322, USA. SN - 0021-9258 N2 - The best understood system for the transport of macromolecules between the cytoplasm and the nucleus is the classical nuclear import pathway. In this pathway, a protein containing a classical basic nuclear localization signal (NLS) is imported by a heterodimeric import receptor consisting of the beta-karyopherin importin beta, which mediates interactions with the nuclear pore complex, and the adaptor protein importin alpha, which directly binds the classical NLS. Here we review recent studies that have advanced our understanding of this pathway and also take a bioinformatics approach to analyze the likely prevalence of this system in vivo. Finally, we describe how a predicted NLS within a protein of interest can be confirmed experimentally to be functionally important. KW - bioinformatics KW - importin KW - nls KW - nuclear KW - review AU - Lange, A AU - Mills, RE AU - Lange, CJ AU - Stewart, M AU - Devine, SE AU - Corbett, AH PY - 2007/02/23/ UR - http://dx.doi.org/10.1074/jbc.R600026200 ER -