Centrosomal anchoring of the protein kinase CK1delta mediated by attachment to the large, coiled-coil scaffolding protein CG-NAP/AKAP450.

@article{Sillibourne2002, abstract = {Protein kinase CK1 (formerly termed casein kinase I) is ubiquitous in eukaryotic cells and comprises a family of as many as 14 isoforms (including splice variants) in mammalian cells. Mammalian CK1delta and CK1epsilon, which are highly related to each other, are enriched at the centrosomes in interphase cells and at the spindle during mitosis. In the present study we have isolated, using the yeast two-hybrid system, a 182 amino acid residue fragment of the centrosomal and golgi N-kinase anchoring protein (CG-NAP, also known as AKAP450), which specifically interacts with CK1delta and CK1epsilon, but not with other CK1 isoforms. The 182 amino acid residue CG-NAP fragment, or full length CG-NAP, co-immunoprecipitates with CK1delta and CK1epsilon from mammalian cells. Consistent with this association, endogenous CG-NAP/AKAP450 and CK1delta co-localize in cells. Moreover, when expressed in the presence of CK1delta the 182 amino acid residue CG-NAP fragment adopts the same sub-cellular localization as CK1delta. Strikingly, attachment of the CG-NAP fragment to the plasma membrane is sufficient to re-localize a significant level of CK1delta to the membrane. These findings support a model in which sub-cellular localization of CK1delta/epsilon molecules at the centrosome is mediated, at least in part, through the action of CG-NAP/AKAP450 and provide a potential mechanism by which the contribution to cell cycle progression by CK1delta/epsilon may be regulated.}, author = {Sillibourne, James E. and Milne, Diane M. and Takahashi, Mikiko and Ono, Yoshitaka and Meek, David W. }, citeulike-article-id = {2054456}, journal = {J Mol Biol}, keywords = {adaptor, aethiops, amino-acid, anchor, animal, article-predikin, carrier, casein, catalysis, cell, centrosome, cercopithecus, cos, cytoskeletal, data, fractions, fragments, fusion, human, isoenzymes, kinase, male, molecular, peptide, precipitin, protein, rat, recombinant, sequence, signal, specificity, structure, subcellular, substrate, tertiary, tests, transducing}, month = {Sep}, number = {4}, pages = {785--797}, posted-at = {2007-12-04 03:22:14}, priority = {2}, title = {Centrosomal anchoring of the protein kinase CK1delta mediated by attachment to the large, coiled-coil scaffolding protein CG-NAP/AKAP450.}, volume = {322}, year = {2002} }

TY - JOUR ID - Sillibourne2002 L3 - citeulike-article-id:2054456 TI - Centrosomal anchoring of the protein kinase CK1delta mediated by attachment to the large, coiled-coil scaffolding protein CG-NAP/AKAP450. JF - J Mol Biol VL - 322 IS - 4 SP - 785 EP - 797 N2 - Protein kinase CK1 (formerly termed casein kinase I) is ubiquitous in eukaryotic cells and comprises a family of as many as 14 isoforms (including splice variants) in mammalian cells. Mammalian CK1delta and CK1epsilon, which are highly related to each other, are enriched at the centrosomes in interphase cells and at the spindle during mitosis. In the present study we have isolated, using the yeast two-hybrid system, a 182 amino acid residue fragment of the centrosomal and golgi N-kinase anchoring protein (CG-NAP, also known as AKAP450), which specifically interacts with CK1delta and CK1epsilon, but not with other CK1 isoforms. The 182 amino acid residue CG-NAP fragment, or full length CG-NAP, co-immunoprecipitates with CK1delta and CK1epsilon from mammalian cells. Consistent with this association, endogenous CG-NAP/AKAP450 and CK1delta co-localize in cells. Moreover, when expressed in the presence of CK1delta the 182 amino acid residue CG-NAP fragment adopts the same sub-cellular localization as CK1delta. Strikingly, attachment of the CG-NAP fragment to the plasma membrane is sufficient to re-localize a significant level of CK1delta to the membrane. These findings support a model in which sub-cellular localization of CK1delta/epsilon molecules at the centrosome is mediated, at least in part, through the action of CG-NAP/AKAP450 and provide a potential mechanism by which the contribution to cell cycle progression by CK1delta/epsilon may be regulated. KW - adaptor KW - aethiops KW - amino-acid KW - anchor KW - animal KW - article-predikin KW - carrier KW - casein KW - catalysis KW - cell KW - centrosome KW - cercopithecus KW - cos KW - cytoskeletal KW - data KW - fractions KW - fragments KW - fusion KW - human KW - isoenzymes KW - kinase KW - male KW - molecular KW - peptide KW - precipitin KW - protein KW - rat KW - recombinant KW - sequence KW - signal KW - specificity KW - structure KW - subcellular KW - substrate KW - tertiary KW - tests KW - transducing AU - Sillibourne, James AU - Milne, Diane AU - Takahashi, Mikiko AU - Ono, Yoshitaka AU - Meek, David PY - 2002/Sep// ER -