Active Ca2+/calmodulin-dependent protein kinase II gamma B impairs positive selection of T cells by modulating TCR signaling.

@article{McGargill2005, abstract = {T cell development is regulated at two critical checkpoints that involve signaling events through the TCR. These signals are propagated by kinases of the Src and Syk families, which activate several adaptor molecules to trigger Ca(2+) release and, in turn, Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) activation. In this study, we show that a constitutively active form of CaMKII antagonizes TCR signaling and impairs positive selection of thymocytes in mice. Following TCR engagement, active CaMKII decreases TCR-mediated CD3zeta chain phosphorylation and ZAP70 recruitment, preventing further downstream events. Therefore, we propose that CaMKII belongs to a negative-feedback loop that modulates the strength of the TCR signal through the tyrosine phosphatase Src homology 2 domain-containing phosphatase 2 (SHP-2).}, author = {Mcgargill, Maureen A. and Sharp, Leslie L. and Bui, Jack D. and Hedrick, Stephen M. and Calbo, S\'{e}bastien }, citeulike-article-id = {2054447}, journal = {J Immunol}, keywords = {animals, antigen, antigens, article-predikin, c57bl, ca2-calmodulin, calcium, cd3, cell, dependent, differentiation, domains, female, fluid, homology, humans, inbred, intracellular, isoenzymes, kinase, knockout, line, lymphocyte, male, mice, p56lck, peptides, phosphorylation, protein, proteins, protein-tyrosine-phosphatase, receptors, signal, signaling, specific, src, subsets, t-cell, t-lymphocyte, transduction, transgenic, tumor, tyrosine}, month = {Jul}, number = {2}, pages = {656--664}, posted-at = {2007-12-04 03:22:14}, priority = {2}, title = {Active Ca2+/calmodulin-dependent protein kinase II gamma B impairs positive selection of T cells by modulating TCR signaling.}, volume = {175}, year = {2005} }

TY - JOUR ID - McGargill2005 L3 - citeulike-article-id:2054447 TI - Active Ca2+/calmodulin-dependent protein kinase II gamma B impairs positive selection of T cells by modulating TCR signaling. JF - J Immunol VL - 175 IS - 2 SP - 656 EP - 664 N2 - T cell development is regulated at two critical checkpoints that involve signaling events through the TCR. These signals are propagated by kinases of the Src and Syk families, which activate several adaptor molecules to trigger Ca(2+) release and, in turn, Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) activation. In this study, we show that a constitutively active form of CaMKII antagonizes TCR signaling and impairs positive selection of thymocytes in mice. Following TCR engagement, active CaMKII decreases TCR-mediated CD3zeta chain phosphorylation and ZAP70 recruitment, preventing further downstream events. Therefore, we propose that CaMKII belongs to a negative-feedback loop that modulates the strength of the TCR signal through the tyrosine phosphatase Src homology 2 domain-containing phosphatase 2 (SHP-2). KW - animals KW - antigen KW - antigens KW - article-predikin KW - c57bl KW - ca2-calmodulin KW - calcium KW - cd3 KW - cell KW - dependent KW - differentiation KW - domains KW - female KW - fluid KW - homology KW - humans KW - inbred KW - intracellular KW - isoenzymes KW - kinase KW - knockout KW - line KW - lymphocyte KW - male KW - mice KW - p56lck KW - peptides KW - phosphorylation KW - protein KW - proteins KW - protein-tyrosine-phosphatase KW - receptors KW - signal KW - signaling KW - specific KW - src KW - subsets KW - t-cell KW - t-lymphocyte KW - transduction KW - transgenic KW - tumor KW - tyrosine AU - Mcgargill, Maureen AU - Sharp, Leslie AU - Bui, Jack AU - Hedrick, Stephen AU - Calbo, Sébastien PY - 2005/Jul// ER -