Proteomic and computational analysis of secreted proteins with type I signal peptides from the Antarctic archaeon Methanococcoides burtonii.

@article{Saunders2006, abstract = {LC-MS/MS was used to identify secreted proteins in the Antarctic archaeon Methanococcoides burtonii. Seven proteins possessing a classical class 1 signal peptide were identified in the supernatant from cultures grown at 4 and 23 degrees C. The proteins included a putative S-layer cell surface protein, cell surface protein involved with cell adhesion, and trypsin-like serine protease. Protease activity was detected in the secreted fraction, and the signal peptide cleavage site of the protease was confirmed using Edman sequencing. The expression profile of putative cell surface proteins suggests a requirement for cell interactions during growth at low temperature. Sequences of the secreted proteins were used to compile a dataset containing a further 32 predicted secreted proteins from the Methanosarcinaceae. Many of these proteins were also S-layer cell surface proteins with a variety of predicted roles, particularly in cell-cell interaction. Computational analysis of signal peptides revealed a preference for lysine in the n-region, leucine in the h-region, and a eucaryal-type cleavage site, highlighting the mosaic nature of signal peptides in Archaea. This is the first study to experimentally characterize secreted proteins from a cold-adapted archaeon and provides new insight and a functional dataset for studying secretion in Archaea.}, author = {Saunders, Neil F. W. and Ng, Charmaine and Raftery, Mark and Guilhaus, Michael and Goodchild, Amber and Cavicchioli, Ricardo }, citeulike-article-id = {1963558}, doi = {10.1021/pr060220x}, journal = {J Proteome Res}, keywords = {alignment, amino-acid, analysis, antarctic, archaea, bibtex-import, biology, chromatography, compounds, computational, data, liquid, mass-spec, methanosarcinaceae, molecular, organophosphorus, protein, proteomics, region, sequence, signal, sorting}, month = {Sep}, number = {9}, pages = {2457--2464}, posted-at = {2007-11-23 05:17:31}, priority = {2}, title = {Proteomic and computational analysis of secreted proteins with type I signal peptides from the Antarctic archaeon Methanococcoides burtonii.}, url = {http://dx.doi.org/10.1021/pr060220x}, volume = {5}, year = {2006} }

TY - JOUR ID - Saunders2006 L3 - citeulike-article-id:1963558 TI - Proteomic and computational analysis of secreted proteins with type I signal peptides from the Antarctic archaeon Methanococcoides burtonii. JF - J Proteome Res VL - 5 IS - 9 SP - 2457 EP - 2464 N2 - LC-MS/MS was used to identify secreted proteins in the Antarctic archaeon Methanococcoides burtonii. Seven proteins possessing a classical class 1 signal peptide were identified in the supernatant from cultures grown at 4 and 23 degrees C. The proteins included a putative S-layer cell surface protein, cell surface protein involved with cell adhesion, and trypsin-like serine protease. Protease activity was detected in the secreted fraction, and the signal peptide cleavage site of the protease was confirmed using Edman sequencing. The expression profile of putative cell surface proteins suggests a requirement for cell interactions during growth at low temperature. Sequences of the secreted proteins were used to compile a dataset containing a further 32 predicted secreted proteins from the Methanosarcinaceae. Many of these proteins were also S-layer cell surface proteins with a variety of predicted roles, particularly in cell-cell interaction. Computational analysis of signal peptides revealed a preference for lysine in the n-region, leucine in the h-region, and a eucaryal-type cleavage site, highlighting the mosaic nature of signal peptides in Archaea. This is the first study to experimentally characterize secreted proteins from a cold-adapted archaeon and provides new insight and a functional dataset for studying secretion in Archaea. KW - alignment KW - amino-acid KW - analysis KW - antarctic KW - archaea KW - bibtex-import KW - biology KW - chromatography KW - compounds KW - computational KW - data KW - liquid KW - mass-spec KW - methanosarcinaceae KW - molecular KW - organophosphorus KW - protein KW - proteomics KW - region KW - sequence KW - signal KW - sorting AU - Saunders, Neil AU - Ng, Charmaine AU - Raftery, Mark AU - Guilhaus, Michael AU - Goodchild, Amber AU - Cavicchioli, Ricardo PY - 2006/Sep// UR - http://dx.doi.org/10.1021/pr060220x ER -