Expression profiling reveals off-target gene regulation by RNAi.

by: AL Jackson, SR Bartz, J Schelter, SV Kobayashi, J Burchard, M Mao, B Li, G Cavet, PS Linsley

Nat Biotechnol, Vol. 21, No. 6. (June 2003), pp. 635-637.

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Abstract

RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity.

@article{citeulike:1287778, abstract = {RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity.}, address = {Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA. aimee\_jackson@merck.com}, author = {Jackson, A. L. and Bartz, S. R. and Schelter, J. and Kobayashi, S. V. and Burchard, J. and Mao, M. and Li, B. and Cavet, G. and Linsley, P. S. }, citeulike-article-id = {1287778}, doi = {http://dx.doi.org/10.1038/nbt831}, issn = {1087-0156}, journal = {Nat Biotechnol}, keywords = {bioinformatics, off-target, sirna}, month = {June}, number = {6}, pages = {635--637}, posted-at = {2007-05-10 09:46:36}, priority = {2}, title = {Expression profiling reveals off-target gene regulation by RNAi.}, url = {http://dx.doi.org/10.1038/nbt831}, volume = {21}, year = {2003} }

RIS record

TY - JOUR ID - citeulike:1287778 L3 - citeulike-article-id:1287778 TI - Expression profiling reveals off-target gene regulation by RNAi. JF - Nat Biotechnol VL - 21 IS - 6 SP - 635 EP - 637 AD - Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA. aimee_jackson@merck.com SN - 1087-0156 N2 - RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity. KW - bioinformatics KW - off-target KW - sirna AU - Jackson, AL AU - Bartz, SR AU - Schelter, J AU - Kobayashi, SV AU - Burchard, J AU - Mao, M AU - Li, B AU - Cavet, G AU - Linsley, PS PY - 2003/06// UR - http://dx.doi.org/10.1038/nbt831 ER -

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