Toward rules relating zinc finger protein sequences and DNA binding site preferences.

@article{desjarlais1998, abstract = {Zinc finger proteins of the Cys2-His2 type consist of tandem arrays of domains, where each domain appears to contact three adjacent base pairs of DNA through three key residues. We have designed and prepared a series of variants of the central zinc finger within the DNA binding domain of Sp1 by using information from an analysis of a large data base of zinc finger protein sequences. Through systematic variations at two of the three contact positions (underlined), relatively specific recognition of sequences of the form 5'-GGGGN(G or T)GGG-3' has been achieved. These results provide the basis for rules that may develop into a code that will allow the design of zinc finger proteins with preselected DNA site specificity.}, address = {Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 20218.}, author = {Desjarlais, J. R. and Berg, J. M. }, citeulike-article-id = {933568}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {zinc\_fingers}, month = {August}, number = {16}, pages = {7345--7349}, posted-at = {2008-05-15 09:56:55}, priority = {2}, title = {Toward rules relating zinc finger protein sequences and DNA binding site preferences.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1502144}, volume = {89}, year = {1992} }

TY - JOUR ID - desjarlais1998 L3 - citeulike-article-id:933568 TI - Toward rules relating zinc finger protein sequences and DNA binding site preferences. JF - Proc Natl Acad Sci U S A VL - 89 IS - 16 SP - 7345 EP - 7349 AD - Thomas C. Jenkins Department of Biophysics, Johns Hopkins University, Baltimore, MD 20218. SN - 0027-8424 N2 - Zinc finger proteins of the Cys2-His2 type consist of tandem arrays of domains, where each domain appears to contact three adjacent base pairs of DNA through three key residues. We have designed and prepared a series of variants of the central zinc finger within the DNA binding domain of Sp1 by using information from an analysis of a large data base of zinc finger protein sequences. Through systematic variations at two of the three contact positions (underlined), relatively specific recognition of sequences of the form 5'-GGGGN(G or T)GGG-3' has been achieved. These results provide the basis for rules that may develop into a code that will allow the design of zinc finger proteins with preselected DNA site specificity. KW - zinc_fingers AU - Desjarlais, JR AU - Berg, JM PY - 1992/08/15/ UR - http://view.ncbi.nlm.nih.gov/pubmed/1502144 ER -