The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release.

@article{citeulike:1531401, abstract = {A deletion mutation affecting vpu was introduced into an infectious molecular clone of human immunodeficiency virus type 1, and the resultant phenotype was examined after infection of human T lymphocytes. The absence of vpu resulted in an accumulation of cell-associated viral proteins and impaired the release of progeny virions. Both electron microscopic and biochemical analyses indicated that a large proportion of the mutant particles was attached to the surface of infected cells. Significant variation in the size and shape of these progeny virions was observed. In addition, intracytoplasmic particles, some of which formed aberrant budding structures, were visualized in T cells infected with the vpu mutant. Indirect immunofluorescence analyses of cultures inoculated with wild-type virus with use of a vpu-specific antiserum demonstrated that vpu is mainly localized to a perinuclear region in the cytoplasm of virus-producing cells.}, address = {Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892.}, author = {Klimkait, T. and Strebel, K. and Hoggan, M. D. and Martin, M. A. and Orenstein, J. M. }, citeulike-article-id = {1531401}, issn = {0022-538X}, journal = {J Virol}, keywords = {binding, gene, hiv, ic, new, site}, month = {February}, number = {2}, pages = {621--629}, posted-at = {2007-08-02 23:21:08}, priority = {2}, title = {The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/2404139}, volume = {64}, year = {1990} }

TY - JOUR ID - citeulike:1531401 L3 - citeulike-article-id:1531401 TI - The human immunodeficiency virus type 1-specific protein vpu is required for efficient virus maturation and release. JF - J Virol VL - 64 IS - 2 SP - 621 EP - 629 AD - Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892. SN - 0022-538X N2 - A deletion mutation affecting vpu was introduced into an infectious molecular clone of human immunodeficiency virus type 1, and the resultant phenotype was examined after infection of human T lymphocytes. The absence of vpu resulted in an accumulation of cell-associated viral proteins and impaired the release of progeny virions. Both electron microscopic and biochemical analyses indicated that a large proportion of the mutant particles was attached to the surface of infected cells. Significant variation in the size and shape of these progeny virions was observed. In addition, intracytoplasmic particles, some of which formed aberrant budding structures, were visualized in T cells infected with the vpu mutant. Indirect immunofluorescence analyses of cultures inoculated with wild-type virus with use of a vpu-specific antiserum demonstrated that vpu is mainly localized to a perinuclear region in the cytoplasm of virus-producing cells. KW - binding KW - gene KW - hiv KW - ic KW - new KW - site AU - Klimkait, T AU - Strebel, K AU - Hoggan, MD AU - Martin, MA AU - Orenstein, JM PY - 1990/02// UR - http://view.ncbi.nlm.nih.gov/pubmed/2404139 ER -