Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow

@article{citeulike:2682989, abstract = {Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of the critical surface concentration of TF required to trigger coagulation under flow. Whole blood supplemented with corn trypsin inhibitor (to inhibit factor XIIa) was perfused over microarrays for 5 minutes. Immunofluorescence staining of platelet glycoprotein GPIb{alpha} and fibrin(ogen) revealed a critical TF concentration (EC50) of 3.6, 8.4, and 10.2 molecules-TF/{micro}m2 at wall shear rates of 100, 500, and 1000 s1, respectively. For collagen arrays where only the center lane of spots (in the direction of flow) contained TF, a downstream distance of 14 mm was required for the thrombus to widen enough to reach across a 300-micrometer gap to the adjacent TF-free lanes of collagen spots, in agreement with numerical simulation. To investigate the effect of low levels of circulating TF, whole blood ({+/-} 100 fM added TF) was tested under static and flow conditions. After 5 minutes, the addition of 100 fM TF to whole blood had negligible effect under static conditions, but caused a 2.5-fold increase in fibrin formation under flow. This report defines the threshold concentrations of surface TF required to trigger coagulation under flow. 10.1182/blood-2007-08-106229}, author = {Okorie, Uzoma M. and Denney, William S. and Chatterjee, Manash S. and Neeves, Keith B. and Diamond, Scott L. }, citeulike-article-id = {2682989}, doi = {10.1182/blood-2007-08-106229}, journal = {Blood}, keywords = {flow, tissue\_factor}, month = {April}, number = {7}, pages = {3507--3513}, posted-at = {2008-04-17 18:17:11}, priority = {3}, title = {Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow}, url = {http://dx.doi.org/10.1182/blood-2007-08-106229}, volume = {111}, year = {2008} }

TY - JOUR ID - citeulike:2682989 L3 - citeulike-article-id:2682989 TI - Determination of surface tissue factor thresholds that trigger coagulation at venous and arterial shear rates: amplification of 100 fM circulating tissue factor requires flow JF - Blood VL - 111 IS - 7 SP - 3507 EP - 3513 N2 - Protein microarrays presenting spots of collagen and lipidated tissue factor (TF) allowed a determination of the critical surface concentration of TF required to trigger coagulation under flow. Whole blood supplemented with corn trypsin inhibitor (to inhibit factor XIIa) was perfused over microarrays for 5 minutes. Immunofluorescence staining of platelet glycoprotein GPIb{alpha} and fibrin(ogen) revealed a critical TF concentration (EC50) of 3.6, 8.4, and 10.2 molecules-TF/{micro}m2 at wall shear rates of 100, 500, and 1000 s1, respectively. For collagen arrays where only the center lane of spots (in the direction of flow) contained TF, a downstream distance of 14 mm was required for the thrombus to widen enough to reach across a 300-micrometer gap to the adjacent TF-free lanes of collagen spots, in agreement with numerical simulation. To investigate the effect of low levels of circulating TF, whole blood ({+/-} 100 fM added TF) was tested under static and flow conditions. After 5 minutes, the addition of 100 fM TF to whole blood had negligible effect under static conditions, but caused a 2.5-fold increase in fibrin formation under flow. This report defines the threshold concentrations of surface TF required to trigger coagulation under flow. 10.1182/blood-2007-08-106229 KW - flow KW - tissue_factor AU - Okorie, Uzoma AU - Denney, William AU - Chatterjee, Manash AU - Neeves, Keith AU - Diamond, Scott PY - 2008/04/01/ UR - http://dx.doi.org/10.1182/blood-2007-08-106229 ER -