Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish

by: Viravuth P Yin, Michael J Thomson, Ryan Thummel, David R Hyde, Scott M Hammond, Kenneth D Poss

Genes Dev., Vol. 22, No. 6. (15 March 2008), pp. 728-733.

View FullText article

DOI, HighWire, Pubmed, Hubmed

Reviews [Write a review of this article]

There are no reviews of this article

Find related articles from these CiteULike users

maralena

Find related articles with these CiteULike tags

fgf, microrna, mir-133, zebrafish

Abstract

Appendage regeneration is defined by rapid changes in gene expression that achieve dramatic developmental effects, suggesting involvement of microRNAs (miRNAs). Here, we find dynamic regulation of many miRNAs during zebrafish fin regeneration. In particular, miR-133 levels are high in uninjured fins but low during regeneration. When regeneration was blocked by Fibroblast growth factor (Fgf) receptor inhibition, high miR-133 levels were quickly restored. Experimentally increasing amounts of miR-133 attenuated fin regeneration. Conversely, miR-133 antagonism during Fgf receptor inhibition accelerated regeneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal proliferation, is an in vivo target of miR-133. Our findings identify miRNA depletion as a new regulatory mechanism for complex tissue regeneration. 10.1101/gad.1641808

@article{citeulike:2798923, abstract = {Appendage regeneration is defined by rapid changes in gene expression that achieve dramatic developmental effects, suggesting involvement of microRNAs (miRNAs). Here, we find dynamic regulation of many miRNAs during zebrafish fin regeneration. In particular, miR-133 levels are high in uninjured fins but low during regeneration. When regeneration was blocked by Fibroblast growth factor (Fgf) receptor inhibition, high miR-133 levels were quickly restored. Experimentally increasing amounts of miR-133 attenuated fin regeneration. Conversely, miR-133 antagonism during Fgf receptor inhibition accelerated regeneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal proliferation, is an in vivo target of miR-133. Our findings identify miRNA depletion as a new regulatory mechanism for complex tissue regeneration. 10.1101/gad.1641808}, author = {Yin, Viravuth P. and Thomson, Michael J. and Thummel, Ryan and Hyde, David R. and Hammond, Scott M. and Poss, Kenneth D. }, citeulike-article-id = {2798923}, doi = {10.1101/gad.1641808}, journal = {Genes Dev.}, keywords = {fgf, microrna, mir-133, zebrafish}, month = {March}, number = {6}, pages = {728--733}, posted-at = {2008-05-14 15:09:43}, priority = {2}, title = {Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish}, url = {http://dx.doi.org/10.1101/gad.1641808}, volume = {22}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2798923 L3 - citeulike-article-id:2798923 TI - Fgf-dependent depletion of microRNA-133 promotes appendage regeneration in zebrafish JF - Genes Dev. VL - 22 IS - 6 SP - 728 EP - 733 N2 - Appendage regeneration is defined by rapid changes in gene expression that achieve dramatic developmental effects, suggesting involvement of microRNAs (miRNAs). Here, we find dynamic regulation of many miRNAs during zebrafish fin regeneration. In particular, miR-133 levels are high in uninjured fins but low during regeneration. When regeneration was blocked by Fibroblast growth factor (Fgf) receptor inhibition, high miR-133 levels were quickly restored. Experimentally increasing amounts of miR-133 attenuated fin regeneration. Conversely, miR-133 antagonism during Fgf receptor inhibition accelerated regeneration through increased proliferation within the regeneration blastema. The Mps1 kinase, an established positive regulator of blastemal proliferation, is an in vivo target of miR-133. Our findings identify miRNA depletion as a new regulatory mechanism for complex tissue regeneration. 10.1101/gad.1641808 KW - fgf KW - microrna KW - mir-133 KW - zebrafish AU - Yin, Viravuth AU - Thomson, Michael AU - Thummel, Ryan AU - Hyde, David AU - Hammond, Scott AU - Poss, Kenneth PY - 2008/03/15/ UR - http://dx.doi.org/10.1101/gad.1641808 ER -

RIS BibTeX