microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells

@article{citeulike:2074199, abstract = {Summary microRNA-155 (miR-155) is expressed by cells of the immune system after activation and has been shown to be required for antibody production after vaccination with attenuated Salmonella. Here we show the intrinsic requirement for miR-155 in B cell responses to thymus-dependent and -independent antigens. B cells lacking miR-155 generated reduced extrafollicular and germinal center responses and failed to produce high-affinity IgG1 antibodies. Gene-expression profiling of activated B cells indicated that miR-155 regulates an array of genes with diverse function, many of which are predicted targets of miR-155. The transcription factor Pu.1 is validated as a direct target of miR155-mediated inhibition. When Pu.1 is overexpressed in wild-type B cells, fewer IgG1 cells are produced, indicating that loss of Pu.1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells.}, author = {Vigorito, Elena and Perks, Kerry L. and Abreu-Goodger, Cei and Bunting, Sam and Xiang, Zou and Kohlhaas, Susan and Das, Partha P. and Miska, Eric A. and Rodriguez, Antony and Bradley, Allan and Smith, Kenneth G. and Rada, Cristina and Enright, Anton J. and Toellner, Kai-Michael and Maclennan, Ian C. and Turner, Martin }, citeulike-article-id = {2074199}, doi = {10.1016/j.immuni.2007.10.009}, journal = {Immunity}, keywords = {b\_cell, bic, igg1, mir-155, pu1}, posted-at = {2008-05-08 12:48:49}, priority = {2}, title = {microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells}, url = {http://dx.doi.org/10.1016/j.immuni.2007.10.009}, volume = {In Press, Corrected Proof} }

TY - JOUR ID - citeulike:2074199 L3 - citeulike-article-id:2074199 TI - microRNA-155 Regulates the Generation of Immunoglobulin Class-Switched Plasma Cells JF - Immunity VL - In Press, Corrected Proof N2 - Summary microRNA-155 (miR-155) is expressed by cells of the immune system after activation and has been shown to be required for antibody production after vaccination with attenuated Salmonella. Here we show the intrinsic requirement for miR-155 in B cell responses to thymus-dependent and -independent antigens. B cells lacking miR-155 generated reduced extrafollicular and germinal center responses and failed to produce high-affinity IgG1 antibodies. Gene-expression profiling of activated B cells indicated that miR-155 regulates an array of genes with diverse function, many of which are predicted targets of miR-155. The transcription factor Pu.1 is validated as a direct target of miR155-mediated inhibition. When Pu.1 is overexpressed in wild-type B cells, fewer IgG1 cells are produced, indicating that loss of Pu.1 regulation is a contributing factor to the miR-155-deficient phenotype. Our results implicate post-transcriptional regulation of gene expression for establishing the terminal differentiation program of B cells. KW - b_cell KW - bic KW - igg1 KW - mir-155 KW - pu1 AU - Vigorito, Elena AU - Perks, Kerry AU - Abreu-Goodger, Cei AU - Bunting, Sam AU - Xiang, Zou AU - Kohlhaas, Susan AU - Das, Partha AU - Miska, Eric AU - Rodriguez, Antony AU - Bradley, Allan AU - Smith, Kenneth AU - Rada, Cristina AU - Enright, Anton AU - Toellner, Kai-Michael AU - Maclennan, Ian AU - Turner, Martin UR - http://dx.doi.org/10.1016/j.immuni.2007.10.009 ER -