A functional study of miR-124 in the developing neural tube.

@article{citeulike:1180909, abstract = {Neural development is a highly orchestrated process that entails precise control of gene expression. Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of individual miRNAs in vertebrate neural development have not been studied in vivo. We investigated the function of the most abundant neuronal miRNA, miR-124, during spinal cord development. Neither inhibition nor overexpression of miR-124 significantly altered the acquisition of neuronal fate, suggesting that miR-124 is unlikely to act as a primary determinant of neuronal differentiation. Two endogenous targets of miR-124, laminin gamma 1 and integrin beta1, were identified, both of which are highly expressed by neural progenitors but repressed upon neuronal differentiation. Thus miR-124 appears to ensure that progenitor genes are post-transcriptionally inhibited in neurons.}, address = {Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA.}, author = {Cao, X. and Pfaff, S. L. and Gage, F. H. }, citeulike-article-id = {1180909}, doi = {10.1101/gad.1519207}, issn = {0890-9369}, journal = {Genes Dev}, keywords = {development, microrna, mir-124}, month = {March}, number = {5}, pages = {531--536}, posted-at = {2008-05-16 14:22:14}, priority = {2}, title = {A functional study of miR-124 in the developing neural tube.}, url = {http://dx.doi.org/10.1101/gad.1519207}, volume = {21}, year = {2007} }

TY - JOUR ID - citeulike:1180909 L3 - citeulike-article-id:1180909 TI - A functional study of miR-124 in the developing neural tube. JF - Genes Dev VL - 21 IS - 5 SP - 531 EP - 536 AD - Laboratory of Genetics, The Salk Institute for Biological Studies, La Jolla, California 92037, USA. SN - 0890-9369 N2 - Neural development is a highly orchestrated process that entails precise control of gene expression. Although microRNAs (miRNAs) have been implicated in fine-tuning gene networks, the roles of individual miRNAs in vertebrate neural development have not been studied in vivo. We investigated the function of the most abundant neuronal miRNA, miR-124, during spinal cord development. Neither inhibition nor overexpression of miR-124 significantly altered the acquisition of neuronal fate, suggesting that miR-124 is unlikely to act as a primary determinant of neuronal differentiation. Two endogenous targets of miR-124, laminin gamma 1 and integrin beta1, were identified, both of which are highly expressed by neural progenitors but repressed upon neuronal differentiation. Thus miR-124 appears to ensure that progenitor genes are post-transcriptionally inhibited in neurons. KW - development KW - microrna KW - mir-124 AU - Cao, X AU - Pfaff, SL AU - Gage, FH PY - 2007/03/01/ UR - http://dx.doi.org/10.1101/gad.1519207 ER -