The Microtubule Plus-End Tracking Protein EB1 Is Required for Kv1 Voltage-Gated K+ Channel Axonal Targeting

@article{citeulike:977948, abstract = {SummaryAxonal Kv1 channels regulate action potential propagation--an evolutionarily conserved function important for the control of motor behavior as evidenced from the linkage of human Kv1 channel mutations to myokymia/episodic ataxia type 1 (EA1) and the Shaker mutant phenotype in Drosophila. To search for the machinery that mediates axonal targeting of Kv1 channels composed of both [alpha] and [beta] subunits, we first demonstrate that Kv[beta]2 is responsible for targeting Kv1 channels to the axon. Next, we show that Kv[beta]2 axonal targeting depends on its ability to associate with the microtubule (MT) plus-end tracking protein (+TIP) EB1. Not only do Kv[beta]2 and EB1 move in unison down the axon, Brefeldin A-sensitive Kv1-containing vesicles can also be found at microtubule ends near the cell membrane. In addition, we found that Kv[beta]2 associates with KIF3/kinesin II as well. Indeed, Kv1 channels rely on both KIF3/kinesin II and EB1 for their axonal targeting.}, author = {Gu, Chen and Zhou, Wei and Puthenveedu, Manojkumar A. and Xu, Mingxuan and Jan, Yuh N. and Jan, Lily Y. }, citeulike-article-id = {977948}, doi = {10.1016/j.neuron.2006.10.022}, journal = {Neuron}, keywords = {axon, cytosqueleton, ion\_channels, kv, targeting, tirf}, month = {December}, number = {5}, pages = {803--816}, posted-at = {2006-12-07 14:14:28}, priority = {0}, title = {The Microtubule Plus-End Tracking Protein EB1 Is Required for Kv1 Voltage-Gated K+ Channel Axonal Targeting}, url = {http://dx.doi.org/10.1016/j.neuron.2006.10.022}, volume = {52}, year = {2006} }

TY - JOUR ID - citeulike:977948 L3 - citeulike-article-id:977948 TI - The Microtubule Plus-End Tracking Protein EB1 Is Required for Kv1 Voltage-Gated K+ Channel Axonal Targeting JF - Neuron VL - 52 IS - 5 SP - 803 EP - 816 N2 - SummaryAxonal Kv1 channels regulate action potential propagation--an evolutionarily conserved function important for the control of motor behavior as evidenced from the linkage of human Kv1 channel mutations to myokymia/episodic ataxia type 1 (EA1) and the Shaker mutant phenotype in Drosophila. To search for the machinery that mediates axonal targeting of Kv1 channels composed of both [alpha] and [beta] subunits, we first demonstrate that Kv[beta]2 is responsible for targeting Kv1 channels to the axon. Next, we show that Kv[beta]2 axonal targeting depends on its ability to associate with the microtubule (MT) plus-end tracking protein (+TIP) EB1. Not only do Kv[beta]2 and EB1 move in unison down the axon, Brefeldin A-sensitive Kv1-containing vesicles can also be found at microtubule ends near the cell membrane. In addition, we found that Kv[beta]2 associates with KIF3/kinesin II as well. Indeed, Kv1 channels rely on both KIF3/kinesin II and EB1 for their axonal targeting. KW - axon KW - cytosqueleton KW - ion_channels KW - kv KW - targeting KW - tirf AU - Gu, Chen AU - Zhou, Wei AU - Puthenveedu, Manojkumar AU - Xu, Mingxuan AU - Jan, Yuh AU - Jan, Lily PY - 2006/12/07/ UR - http://dx.doi.org/10.1016/j.neuron.2006.10.022 ER -