Cargo regulates clathrin-coated pit dynamics.

@article{citeulike:918605, abstract = {Clathrin-coated pits (CCPs) are generally considered a uniform population of endocytic machines containing mixed constitutive and regulated membrane cargo. Contrary to this view, we show that regulated endocytosis of G protein-coupled receptors (GPCRs) occurs preferentially through a subset of CCPs. Significantly, GPCR-containing CCPs are also functionally distinct, as their surface residence time is regulated locally by GPCR cargo via PDZ-dependent linkage to the actin cytoskeleton. Such cargo-regulated CCPs show delayed recruitment of dynamin and can undergo an abortive event in which clathrin coats separate from the plasma membrane without concomitant receptor endocytosis. Segregation of cargo into CCP subsets, combined with cargo-dependent control of CCP dynamics, suggests a simple kinetic mechanism to generate functional specialization early in the endocytic pathway and reduce competition between diverse endocytic cargo.}, address = {Department of Psychiatry, University of California at San Francisco, 600 16th Street, San Francisco, CA 94158, USA.}, author = {Puthenveedu, M. A. and von Zastrow, M. }, citeulike-article-id = {918605}, doi = {10.1016/j.cell.2006.08.035}, issn = {0092-8674}, journal = {Cell}, keywords = {clathrin, endocytosis, tirf}, month = {October}, number = {1}, pages = {113--124}, posted-at = {2006-10-30 16:10:45}, priority = {0}, title = {Cargo regulates clathrin-coated pit dynamics.}, url = {http://dx.doi.org/10.1016/j.cell.2006.08.035}, volume = {127}, year = {2006} }

TY - JOUR ID - citeulike:918605 L3 - citeulike-article-id:918605 TI - Cargo regulates clathrin-coated pit dynamics. JF - Cell VL - 127 IS - 1 SP - 113 EP - 124 AD - Department of Psychiatry, University of California at San Francisco, 600 16th Street, San Francisco, CA 94158, USA. SN - 0092-8674 N2 - Clathrin-coated pits (CCPs) are generally considered a uniform population of endocytic machines containing mixed constitutive and regulated membrane cargo. Contrary to this view, we show that regulated endocytosis of G protein-coupled receptors (GPCRs) occurs preferentially through a subset of CCPs. Significantly, GPCR-containing CCPs are also functionally distinct, as their surface residence time is regulated locally by GPCR cargo via PDZ-dependent linkage to the actin cytoskeleton. Such cargo-regulated CCPs show delayed recruitment of dynamin and can undergo an abortive event in which clathrin coats separate from the plasma membrane without concomitant receptor endocytosis. Segregation of cargo into CCP subsets, combined with cargo-dependent control of CCP dynamics, suggests a simple kinetic mechanism to generate functional specialization early in the endocytic pathway and reduce competition between diverse endocytic cargo. KW - clathrin KW - endocytosis KW - tirf AU - Puthenveedu, MA AU - von Zastrow, M PY - 2006/10/06/ UR - http://dx.doi.org/10.1016/j.cell.2006.08.035 ER -