Rab5 and Rab7 Control Endocytic Sorting along the Axonal Retrograde Transport Pathway

@article{citeulike:906034, abstract = {SummaryVesicular pathways coupling the neuromuscular junction with the motor neuron soma are essential for neuronal function and survival. To characterize the organelles responsible for this long-distance crosstalk, we developed a purification strategy based on a fragment of tetanus neurotoxin (TeNT HC) conjugated to paramagnetic beads. This approach enabled us to identify, among other factors, the small GTPase Rab7 as a functional marker of a specific pool of axonal retrograde carriers, which transport neurotrophins and their receptors. Furthermore, Rab5 is essential for an early step in TeNT HC sorting but is absent from axonally transported vesicles. Our data demonstrate that TeNT HC uses a retrograde transport pathway shared with p75NTR, TrkB, and BDNF, which is strictly dependent on the activities of both Rab5 and Rab7. Therefore, Rab7 plays an essential role in axonal retrograde transport by controlling a vesicular compartment implicated in neurotrophin traffic.}, author = {Deinhardt, Katrin and Salinas, Sara and Verastegui, Carole and Watson, Rose and Worth, Daniel and Hanrahan, Sarah and Bucci, Cecilia and Schiavo, Giampietro }, citeulike-article-id = {906034}, doi = {10.1016/j.neuron.2006.08.018}, journal = {Neuron}, keywords = {axon, endocytosis, endosomes, live\_cell\_imaging, neurons, rab, trafficking}, month = {October}, number = {2}, pages = {293--305}, posted-at = {2006-10-19 17:30:14}, priority = {0}, title = {Rab5 and Rab7 Control Endocytic Sorting along the Axonal Retrograde Transport Pathway}, url = {http://dx.doi.org/10.1016/j.neuron.2006.08.018}, volume = {52}, year = {2006} }

TY - JOUR ID - citeulike:906034 L3 - citeulike-article-id:906034 TI - Rab5 and Rab7 Control Endocytic Sorting along the Axonal Retrograde Transport Pathway JF - Neuron VL - 52 IS - 2 SP - 293 EP - 305 N2 - SummaryVesicular pathways coupling the neuromuscular junction with the motor neuron soma are essential for neuronal function and survival. To characterize the organelles responsible for this long-distance crosstalk, we developed a purification strategy based on a fragment of tetanus neurotoxin (TeNT HC) conjugated to paramagnetic beads. This approach enabled us to identify, among other factors, the small GTPase Rab7 as a functional marker of a specific pool of axonal retrograde carriers, which transport neurotrophins and their receptors. Furthermore, Rab5 is essential for an early step in TeNT HC sorting but is absent from axonally transported vesicles. Our data demonstrate that TeNT HC uses a retrograde transport pathway shared with p75NTR, TrkB, and BDNF, which is strictly dependent on the activities of both Rab5 and Rab7. Therefore, Rab7 plays an essential role in axonal retrograde transport by controlling a vesicular compartment implicated in neurotrophin traffic. KW - axon KW - endocytosis KW - endosomes KW - live_cell_imaging KW - neurons KW - rab KW - trafficking AU - Deinhardt, Katrin AU - Salinas, Sara AU - Verastegui, Carole AU - Watson, Rose AU - Worth, Daniel AU - Hanrahan, Sarah AU - Bucci, Cecilia AU - Schiavo, Giampietro PY - 2006/10/19/ UR - http://dx.doi.org/10.1016/j.neuron.2006.08.018 ER -