Role of the PAR-3-KIF3 complex in the establishment of neuronal polarity.

@article{citeulike:768763, abstract = {Neurons polarize to form elaborate multiple dendrites and one long axon. The establishment and maintenance of axon/dendrite polarity are fundamentally important for neurons. Recent studies have demonstrated that the polarity complex PAR-3-PAR-6-atypical protein kinase C (aPKC) is involved in polarity determination in many tissues and cells. The function of the PAR-3-PAR-6-aPKC protein complex depends on its subcellular localization in polarized cells. PAR-3 accumulates at the tip of growing axons in cultured rat hippocampal neurons, but the molecular mechanism of this localization remains unknown. Here we identify a direct interaction between PAR-3 and KIF3A, a plus-end-directed microtubule motor protein, and show that aPKC can associate with KIF3A through its interaction with PAR-3. The expression of dominant-negative PAR-3 and KIF3A fragments that disrupt PAR-3-KIF3A binding inhibited the accumulation of PAR-3 and aPKC at the tip of the neurites and abolished neuronal polarity. These results suggest that PAR-3 is transported to the distal tip of the axon by KIF3A and that the proper localization of PAR-3 is required to establish neuronal polarity.}, address = {Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Showa, Nagoya, Aichi 466-8550, Japan.}, author = {Nishimura, T. and Kato, K. and Yamaguchi, T. and Fukata, Y. and Ohno, S. and Kaibuchi, K. }, citeulike-article-id = {768763}, doi = {10.1038/ncb1118}, issn = {1465-7392}, journal = {Nat Cell Biol}, keywords = {axon, dendrites, motor\_proteins, polarity}, month = {April}, number = {4}, pages = {328--334}, posted-at = {2006-07-21 18:03:56}, priority = {0}, title = {Role of the PAR-3-KIF3 complex in the establishment of neuronal polarity.}, url = {http://dx.doi.org/10.1038/ncb1118}, volume = {6}, year = {2004} }

TY - JOUR ID - citeulike:768763 L3 - citeulike-article-id:768763 TI - Role of the PAR-3-KIF3 complex in the establishment of neuronal polarity. JF - Nat Cell Biol VL - 6 IS - 4 SP - 328 EP - 334 AD - Department of Cell Pharmacology, Graduate School of Medicine, Nagoya University, 65 Tsurumai, Showa, Nagoya, Aichi 466-8550, Japan. SN - 1465-7392 N2 - Neurons polarize to form elaborate multiple dendrites and one long axon. The establishment and maintenance of axon/dendrite polarity are fundamentally important for neurons. Recent studies have demonstrated that the polarity complex PAR-3-PAR-6-atypical protein kinase C (aPKC) is involved in polarity determination in many tissues and cells. The function of the PAR-3-PAR-6-aPKC protein complex depends on its subcellular localization in polarized cells. PAR-3 accumulates at the tip of growing axons in cultured rat hippocampal neurons, but the molecular mechanism of this localization remains unknown. Here we identify a direct interaction between PAR-3 and KIF3A, a plus-end-directed microtubule motor protein, and show that aPKC can associate with KIF3A through its interaction with PAR-3. The expression of dominant-negative PAR-3 and KIF3A fragments that disrupt PAR-3-KIF3A binding inhibited the accumulation of PAR-3 and aPKC at the tip of the neurites and abolished neuronal polarity. These results suggest that PAR-3 is transported to the distal tip of the axon by KIF3A and that the proper localization of PAR-3 is required to establish neuronal polarity. KW - axon KW - dendrites KW - motor_proteins KW - polarity AU - Nishimura, T AU - Kato, K AU - Yamaguchi, T AU - Fukata, Y AU - Ohno, S AU - Kaibuchi, K PY - 2004/04// UR - http://dx.doi.org/10.1038/ncb1118 ER -