Intracellular organization of hippocampal neurons during the development of neuronal polarity.

@article{citeulike:766749, abstract = {In culture, hippocampal neurons initially establish several short, apparently identical processes; of these, only one acquires axonal characteristics, the remainder becoming dendrites. We examined the organization of cytoplasmic constituents that might influence which of the initial processes becomes the axon. The Golgi complex was visualized using either fluorescent wheat germ agglutinin or a specific antibody. Presumptive microtubule-organizing centers were identified by depolymerizing microtubules with nocodazole, then allowing them to repolymerize for brief periods. As judged by light microscopy, hippocampal neurons contained a single Golgi region and a single microtubule-organizing center, which were frequently localized together adjacent to a shallow indentation in the nucleus. In cells fixed shortly after the axons had emerged, there was no correlation between the position of the Golgi complex or the microtubule organizing center and the site of origin of the axon. Based on nuclear shape, the position of the Golgi complex and microtubule-organizing center could also be inferred in living cells. When axonal outgrowth was followed in individual cells by time-lapse microscopy, so that the location of the Golgi complex and microtubule-organizing center could be determined at the exact moment when the axon emerged, no correlation was apparent. Antibodies that recognize specific posttranslational modifications of alpha-tubulin--acetylation and de-tyrosination--were used to assess the distribution of arrays of stable microtubules. Stable microtubules were present in all processes, both before and after the emergence of the axon. They were not confined to the axon. Thus the localization of these cellular constituents does not play a major role in determining which of the processes initially extended by hippocampal neurons becomes the definitive axon.}, address = {European Molecular Biology Laboratory, Heidelberg, FRG.}, author = {Dotti, C. G. and Banker, G. }, citeulike-article-id = {766749}, issn = {0269-3518}, journal = {J Cell Sci Suppl}, keywords = {intracellular, polarity, re\_golgi}, pages = {75--84}, posted-at = {2006-07-20 16:34:27}, priority = {0}, title = {Intracellular organization of hippocampal neurons during the development of neuronal polarity.}, url = {http://view.ncbi.nlm.nih.gov/pubmed/1824109}, volume = {15}, year = {1991} }

TY - JOUR ID - citeulike:766749 L3 - citeulike-article-id:766749 TI - Intracellular organization of hippocampal neurons during the development of neuronal polarity. JF - J Cell Sci Suppl VL - 15 SP - 75 EP - 84 AD - European Molecular Biology Laboratory, Heidelberg, FRG. SN - 0269-3518 N2 - In culture, hippocampal neurons initially establish several short, apparently identical processes; of these, only one acquires axonal characteristics, the remainder becoming dendrites. We examined the organization of cytoplasmic constituents that might influence which of the initial processes becomes the axon. The Golgi complex was visualized using either fluorescent wheat germ agglutinin or a specific antibody. Presumptive microtubule-organizing centers were identified by depolymerizing microtubules with nocodazole, then allowing them to repolymerize for brief periods. As judged by light microscopy, hippocampal neurons contained a single Golgi region and a single microtubule-organizing center, which were frequently localized together adjacent to a shallow indentation in the nucleus. In cells fixed shortly after the axons had emerged, there was no correlation between the position of the Golgi complex or the microtubule organizing center and the site of origin of the axon. Based on nuclear shape, the position of the Golgi complex and microtubule-organizing center could also be inferred in living cells. When axonal outgrowth was followed in individual cells by time-lapse microscopy, so that the location of the Golgi complex and microtubule-organizing center could be determined at the exact moment when the axon emerged, no correlation was apparent. Antibodies that recognize specific posttranslational modifications of alpha-tubulin--acetylation and de-tyrosination--were used to assess the distribution of arrays of stable microtubules. Stable microtubules were present in all processes, both before and after the emergence of the axon. They were not confined to the axon. Thus the localization of these cellular constituents does not play a major role in determining which of the processes initially extended by hippocampal neurons becomes the definitive axon. KW - intracellular KW - polarity KW - re_golgi AU - Dotti, CG AU - Banker, G PY - 1991/// UR - http://view.ncbi.nlm.nih.gov/pubmed/1824109 ER -