Visualization of molecular interactions by fluorescence complementation

by: Tom K Kerppola

Nature Reviews Molecular Cell Biology, Vol. 7, No. 6. (19 April 2006), pp. 449-456.

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Abstract

The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.

@article{citeulike:594916, abstract = {The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides.}, address = {Tom K. Kerppola is at the Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA. kerppola@umich.edu.}, author = {Kerppola, Tom K. }, citeulike-article-id = {594916}, doi = {10.1038/nrm1929}, issn = {1471-0072}, journal = {Nature Reviews Molecular Cell Biology}, keywords = {bifc, microscopy, review}, month = {April}, number = {6}, pages = {449--456}, posted-at = {2008-03-21 14:31:05}, priority = {0}, publisher = {Nature Publishing Group}, title = {Visualization of molecular interactions by fluorescence complementation}, url = {http://dx.doi.org/10.1038/nrm1929}, volume = {7}, year = {2006} }

RIS record

TY - JOUR ID - citeulike:594916 L3 - citeulike-article-id:594916 TI - Visualization of molecular interactions by fluorescence complementation JF - Nature Reviews Molecular Cell Biology VL - 7 IS - 6 SP - 449 EP - 456 AD - Tom K. Kerppola is at the Howard Hughes Medical Institute and Department of Biological Chemistry, University of Michigan Medical School, Ann Arbor, Michigan 48109-0650, USA. kerppola@umich.edu. PB - Nature Publishing Group SN - 1471-0072 N2 - The visualization of protein complexes in living cells enables the examination of protein interactions in their normal environment and the determination of their subcellular localization. The bimolecular fluorescence complementation assay has been used to visualize interactions among multiple proteins in many cell types and organisms. Modified forms of this assay have been used to visualize the competition between alternative interaction partners and the covalent modification of proteins by ubiquitin-family peptides. KW - bifc KW - microscopy KW - review AU - Kerppola, Tom PY - 2006/04/19/ UR - http://dx.doi.org/10.1038/nrm1929 ER -

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