AMPA-receptor activation regulates the diffusion of a membrane marker in parallel with dendritic spine motility in the mouse hippocampus.

@article{citeulike:3080826, abstract = {Dendritic spines are the site of most excitatory connections in the hippocampus. We have investigated the diffusibility of a membrane-bound green fluorescent protein (mGFP) within the inner leaflet of the plasma membrane using Fluorescence Recovery After Photobleaching. In dendritic spines the diffusion of mGFP was significantly retarded relative to the dendritic shaft. In parallel, we have assessed the motility of dendritic spines, and found an inverse correlation between spine motility and the rate of diffusion of mGFP. We then tested the influence of glutamate receptor activation or blockade, and the involvement of the actin cytoskeleton (using latrunculin A) on spine motility and mGFP diffusion. These results show that glutamate receptors regulate the mobility of molecules in the inner leaflet of the plasma membrane through an action upon the actin cytoskeleton, suggesting a novel mechanism for the regulation of postsynaptic receptor density and composition.}, address = {Brain Research Institute, University of Zurich, Zurich, Switzerland. richards@physiology.wisc.edu}, author = {Richards, D. A. and De Paola, V. and Caroni, P. and G\"{a}hwiler, B. H. and McKinney, R. A. }, citeulike-article-id = {3080826}, doi = {http://dx.doi.org/10.1113/jphysiol.2004.062091}, issn = {0022-3751}, journal = {The Journal of physiology}, keywords = {ampar, dendritic\_spines, frap, membrane\_diffusion, slice\_culture}, month = {July}, number = {Pt 2}, pages = {503--512}, posted-at = {2008-08-04 13:38:31}, priority = {0}, title = {AMPA-receptor activation regulates the diffusion of a membrane marker in parallel with dendritic spine motility in the mouse hippocampus.}, url = {http://dx.doi.org/10.1113/jphysiol.2004.062091}, volume = {558}, year = {2004} }

TY - JOUR ID - citeulike:3080826 L3 - citeulike-article-id:3080826 TI - AMPA-receptor activation regulates the diffusion of a membrane marker in parallel with dendritic spine motility in the mouse hippocampus. JF - The Journal of physiology VL - 558 IS - Pt 2 SP - 503 EP - 512 AD - Brain Research Institute, University of Zurich, Zurich, Switzerland. richards@physiology.wisc.edu SN - 0022-3751 N2 - Dendritic spines are the site of most excitatory connections in the hippocampus. We have investigated the diffusibility of a membrane-bound green fluorescent protein (mGFP) within the inner leaflet of the plasma membrane using Fluorescence Recovery After Photobleaching. In dendritic spines the diffusion of mGFP was significantly retarded relative to the dendritic shaft. In parallel, we have assessed the motility of dendritic spines, and found an inverse correlation between spine motility and the rate of diffusion of mGFP. We then tested the influence of glutamate receptor activation or blockade, and the involvement of the actin cytoskeleton (using latrunculin A) on spine motility and mGFP diffusion. These results show that glutamate receptors regulate the mobility of molecules in the inner leaflet of the plasma membrane through an action upon the actin cytoskeleton, suggesting a novel mechanism for the regulation of postsynaptic receptor density and composition. KW - ampar KW - dendritic_spines KW - frap KW - membrane_diffusion KW - slice_culture AU - Richards, DA AU - De Paola, V AU - Caroni, P AU - Gähwiler, BH AU - McKinney, RA PY - 2004/07/15/ UR - http://dx.doi.org/10.1113/jphysiol.2004.062091 ER -