Intraneuronal vesicular organelle transport changes with cell population density in vitro.

@article{citeulike:3036319, abstract = {Primary neuron cultures are widely used in research due to the ease and usefulness of observing individual cells. Therefore, it is vital to understand how variations in culture conditions may affect neuron physiology. One potential variation for cultured neurons is a change in intracellular transport. As transport is necessary for the normal delivery of organelles, proteins, nucleic acids, and lipids, it is a logical indicator of a cell's physiology. We test the hypothesis that organelle transport may change with varying in vitro population densities, thus indicating a change in cellular physiology. Using a novel background subtraction imaging method we show that, at 5 days in vitro (DIV), transport of vesicular organelles in embryonic rat spinal cord neurons is positively correlated with cell density. When density increased 6.5-fold, the number of transported organelles increased 2.2+/-0.3-fold. Intriguingly, this effect was not observable at 3-4 DIV. These results show a significant change in cellular physiology with a relatively small change in plating procedure; this indicates that cells appearing to be morphologically similar, and at the same DIV, may still suffer from a great degree of variability.}, address = {Physics Department, Wake Forest University, Winston-Salem, NC 27109-7507, USA.}, author = {Bauer, C. T. and Shtridelman, Y. and Tom\'{e}, C. M. and Grim, J. Q. and Turner, C. P. and Tytell, M. and Macosko, J. C. }, citeulike-article-id = {3036319}, doi = {http://dx.doi.org/10.1016/j.neulet.2008.06.026}, issn = {0304-3940}, journal = {Neuroscience letters}, keywords = {caution, cell\_culture, neurons, trafficking}, month = {August}, number = {2}, pages = {173--177}, posted-at = {2008-07-23 09:07:03}, priority = {0}, title = {Intraneuronal vesicular organelle transport changes with cell population density in vitro.}, url = {http://dx.doi.org/10.1016/j.neulet.2008.06.026}, volume = {441}, year = {2008} }

TY - JOUR ID - citeulike:3036319 L3 - citeulike-article-id:3036319 TI - Intraneuronal vesicular organelle transport changes with cell population density in vitro. JF - Neuroscience letters VL - 441 IS - 2 SP - 173 EP - 177 AD - Physics Department, Wake Forest University, Winston-Salem, NC 27109-7507, USA. SN - 0304-3940 N2 - Primary neuron cultures are widely used in research due to the ease and usefulness of observing individual cells. Therefore, it is vital to understand how variations in culture conditions may affect neuron physiology. One potential variation for cultured neurons is a change in intracellular transport. As transport is necessary for the normal delivery of organelles, proteins, nucleic acids, and lipids, it is a logical indicator of a cell's physiology. We test the hypothesis that organelle transport may change with varying in vitro population densities, thus indicating a change in cellular physiology. Using a novel background subtraction imaging method we show that, at 5 days in vitro (DIV), transport of vesicular organelles in embryonic rat spinal cord neurons is positively correlated with cell density. When density increased 6.5-fold, the number of transported organelles increased 2.2+/-0.3-fold. Intriguingly, this effect was not observable at 3-4 DIV. These results show a significant change in cellular physiology with a relatively small change in plating procedure; this indicates that cells appearing to be morphologically similar, and at the same DIV, may still suffer from a great degree of variability. KW - caution KW - cell_culture KW - neurons KW - trafficking AU - Bauer, CT AU - Shtridelman, Y AU - Tomé, CM AU - Grim, JQ AU - Turner, CP AU - Tytell, M AU - Macosko, JC PY - 2008/08/22/ UR - http://dx.doi.org/10.1016/j.neulet.2008.06.026 ER -