Single-molecule diffusion study of activated EGFR implicates its endocytic pathway.

@article{citeulike:2789783, abstract = {In this work, we have imaged the lateral diffusion of activated epidermal growth factor receptor (EGFR) on cell membrane for studying its internalization pathway. After EGF activation, the mobility of individual EGFR molecules was measured and compared with that in the cells disrupted of clathrin-coated pits and caveolae, the two endocytosis-competent membrane microdomains. The results implicated that activated EGFR molecules associated with clathrin-coated pits but not caveolae at low doses of EGF, whereas they were located in these two domains at high EGF doses. It provided supporting evidence for the occurrence of both clathrin-dependent and caveolae-dependent EGFR endocytosis.}, address = {Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China.}, author = {Xiao, Z. and Zhang, W. and Yang, Y. and Xu, L. and Fang, X. }, citeulike-article-id = {2789783}, doi = {10.1016/j.bbrc.2008.02.084}, issn = {1090-2104}, journal = {Biochemical and biophysical research communications}, keywords = {clathrin, endocytosis, membrane\_diffusion, quantum\_dots, spt, tracking}, month = {May}, number = {2}, pages = {730--734}, posted-at = {2008-05-12 16:33:43}, priority = {0}, title = {Single-molecule diffusion study of activated EGFR implicates its endocytic pathway.}, url = {http://dx.doi.org/10.1016/j.bbrc.2008.02.084}, volume = {369}, year = {2008} }

TY - JOUR ID - citeulike:2789783 L3 - citeulike-article-id:2789783 TI - Single-molecule diffusion study of activated EGFR implicates its endocytic pathway. JF - Biochemical and biophysical research communications VL - 369 IS - 2 SP - 730 EP - 734 AD - Beijing National Laboratory for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, China. SN - 1090-2104 N2 - In this work, we have imaged the lateral diffusion of activated epidermal growth factor receptor (EGFR) on cell membrane for studying its internalization pathway. After EGF activation, the mobility of individual EGFR molecules was measured and compared with that in the cells disrupted of clathrin-coated pits and caveolae, the two endocytosis-competent membrane microdomains. The results implicated that activated EGFR molecules associated with clathrin-coated pits but not caveolae at low doses of EGF, whereas they were located in these two domains at high EGF doses. It provided supporting evidence for the occurrence of both clathrin-dependent and caveolae-dependent EGFR endocytosis. KW - clathrin KW - endocytosis KW - membrane_diffusion KW - quantum_dots KW - spt KW - tracking AU - Xiao, Z AU - Zhang, W AU - Yang, Y AU - Xu, L AU - Fang, X PY - 2008/05/02/ UR - http://dx.doi.org/10.1016/j.bbrc.2008.02.084 ER -