Vaccinia Virus Uses Macropinocytosis and Apoptotic Mimicry to Enter Host Cells

by: Jason Mercer, Ari Helenius

Science, Vol. 320, No. 5875. (25 April 2008), pp. 531-535.

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Abstract

Viruses employ many different strategies to enter host cells. Vaccinia virus, a prototype poxvirus, enters cells in a pH-dependent fashion. Live cell imaging showed that fluorescent virus particles associated with and moved along filopodia to the cell body, where they were internalized after inducing the extrusion of large transient membrane blebs. p21-activated kinase 1 (PAK1) was activated by the virus, and the endocytic process had the general characteristics of macropinocytosis. The induction of blebs, the endocytic event, and infection were all critically dependent on the presence of exposed phosphatidylserine in the viral membrane, which suggests that vaccinia virus uses apoptotic mimicry to enter cells. 10.1126/science.1155164

@article{citeulike:2718925, abstract = {Viruses employ many different strategies to enter host cells. Vaccinia virus, a prototype poxvirus, enters cells in a pH-dependent fashion. Live cell imaging showed that fluorescent virus particles associated with and moved along filopodia to the cell body, where they were internalized after inducing the extrusion of large transient membrane blebs. p21-activated kinase 1 (PAK1) was activated by the virus, and the endocytic process had the general characteristics of macropinocytosis. The induction of blebs, the endocytic event, and infection were all critically dependent on the presence of exposed phosphatidylserine in the viral membrane, which suggests that vaccinia virus uses apoptotic mimicry to enter cells. 10.1126/science.1155164}, author = {Mercer, Jason and Helenius, Ari }, citeulike-article-id = {2718925}, doi = {10.1126/science.1155164}, journal = {Science}, keywords = {cell\_culture, endocytosis, virus\_entry}, month = {April}, number = {5875}, pages = {531--535}, posted-at = {2008-04-30 10:52:31}, priority = {0}, title = {Vaccinia Virus Uses Macropinocytosis and Apoptotic Mimicry to Enter Host Cells}, url = {http://dx.doi.org/10.1126/science.1155164}, volume = {320}, year = {2008} }

RIS record

TY - JOUR ID - citeulike:2718925 TI - Vaccinia Virus Uses Macropinocytosis and Apoptotic Mimicry to Enter Host Cells JF - Science VL - 320 IS - 5875 SP - 531 EP - 535 N2 - Viruses employ many different strategies to enter host cells. Vaccinia virus, a prototype poxvirus, enters cells in a pH-dependent fashion. Live cell imaging showed that fluorescent virus particles associated with and moved along filopodia to the cell body, where they were internalized after inducing the extrusion of large transient membrane blebs. p21-activated kinase 1 (PAK1) was activated by the virus, and the endocytic process had the general characteristics of macropinocytosis. The induction of blebs, the endocytic event, and infection were all critically dependent on the presence of exposed phosphatidylserine in the viral membrane, which suggests that vaccinia virus uses apoptotic mimicry to enter cells. 10.1126/science.1155164 KW - cell_culture KW - endocytosis KW - virus_entry AU - Mercer, Jason AU - Helenius, Ari PY - 2008/04/25/ UR - http://dx.doi.org/10.1126/science.1155164 ER -

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