Culturing hippocampal neurons.

@article{citeulike:2696718, abstract = {We provide protocols for preparing low-density dissociated-cell cultures of hippocampal neurons from embryonic rats or mice. The neurons are cultured on polylysine-treated coverslips, which are suspended above an astrocyte feeder layer and maintained in serum-free medium. When cultured according to this protocol, hippocampal neurons become appropriately polarized, develop extensive axonal and dendritic arbors and form numerous, functional synaptic connections with one another. Hippocampal cultures have been used widely for visualizing the subcellular localization of endogenous or expressed proteins, for imaging protein trafficking and for defining the molecular mechanisms underlying the development of neuronal polarity, dendritic growth and synapse formation. Preparation of glial feeder cultures must begin 2 weeks in advance, and it takes 5 d to prepare coverslips as a substrate for neuronal growth. Dissecting the hippocampus and plating hippocampal neurons takes 2-3 h.}, address = {Center for Research on Occupational and Environmental Toxicology, Oregon Health \& Science University, L606, 3181 SW Sam Jackson Park Rd., Portland, Oregon 97239, USA.}, author = {Kaech, S. and Banker, G. }, citeulike-article-id = {2696718}, doi = {10.1038/nprot.2006.356}, issn = {1750-2799}, journal = {Nature protocols}, keywords = {cultured\_hippo\_neurons, protocol, transfection}, number = {5}, pages = {2406--2415}, posted-at = {2008-04-21 15:16:54}, priority = {0}, title = {Culturing hippocampal neurons.}, url = {http://dx.doi.org/10.1038/nprot.2006.356}, volume = {1}, year = {2006} }

TY - JOUR ID - citeulike:2696718 L3 - citeulike-article-id:2696718 TI - Culturing hippocampal neurons. JF - Nature protocols VL - 1 IS - 5 SP - 2406 EP - 2415 AD - Center for Research on Occupational and Environmental Toxicology, Oregon Health & Science University, L606, 3181 SW Sam Jackson Park Rd., Portland, Oregon 97239, USA. SN - 1750-2799 N2 - We provide protocols for preparing low-density dissociated-cell cultures of hippocampal neurons from embryonic rats or mice. The neurons are cultured on polylysine-treated coverslips, which are suspended above an astrocyte feeder layer and maintained in serum-free medium. When cultured according to this protocol, hippocampal neurons become appropriately polarized, develop extensive axonal and dendritic arbors and form numerous, functional synaptic connections with one another. Hippocampal cultures have been used widely for visualizing the subcellular localization of endogenous or expressed proteins, for imaging protein trafficking and for defining the molecular mechanisms underlying the development of neuronal polarity, dendritic growth and synapse formation. Preparation of glial feeder cultures must begin 2 weeks in advance, and it takes 5 d to prepare coverslips as a substrate for neuronal growth. Dissecting the hippocampus and plating hippocampal neurons takes 2-3 h. KW - cultured_hippo_neurons KW - protocol KW - transfection AU - Kaech, S AU - Banker, G PY - 2006/// UR - http://dx.doi.org/10.1038/nprot.2006.356 ER -