The Subspine Organization of Actin Fibers Regulates the Structure and Plasticity of Dendritic Spines

@article{citeulike:2669700, abstract = {Summary Synapse function and plasticity depend on the physical structure of dendritic spines as determined by the actin cytoskeleton. We have investigated the organization of filamentous (F-) actin within individual spines on CA1 pyramidal neurons in rat hippocampal slices. Using two-photon photoactivation of green fluorescent protein fused to [beta]-actin, we found that a dynamic pool of F-actin at the tip of the spine quickly treadmilled to generate an expansive force. The size of a stable F-actin pool at the base of the spine depended on spine volume. Repeated two-photon uncaging of glutamate formed a third pool of F-actin and enlarged the spine. The spine often released this "enlargement pool" into the dendritic shaft, but the pool had to be physically confined by a spine neck for the enlargement to be long-lasting. Ca2+/calmodulin-dependent protein kinase II regulated this confinement. Thus, spines have an elaborate mechanical nature that is regulated by actin fibers.}, author = {Honkura, Naoki and Matsuzaki, Masanori and Noguchi, Jun and Ellis-Davies, Graham C. and Kasai, Haruo }, citeulike-article-id = {2669700}, doi = {10.1016/j.neuron.2008.01.013}, journal = {Neuron}, keywords = {actin, cytosqueleton, dendritic\_spines, photoactivation}, month = {March}, number = {5}, pages = {719--729}, posted-at = {2008-04-23 10:01:45}, priority = {0}, title = {The Subspine Organization of Actin Fibers Regulates the Structure and Plasticity of Dendritic Spines}, url = {http://dx.doi.org/10.1016/j.neuron.2008.01.013}, volume = {57}, year = {2008} }

TY - JOUR ID - citeulike:2669700 TI - The Subspine Organization of Actin Fibers Regulates the Structure and Plasticity of Dendritic Spines JF - Neuron VL - 57 IS - 5 SP - 719 EP - 729 N2 - Summary Synapse function and plasticity depend on the physical structure of dendritic spines as determined by the actin cytoskeleton. We have investigated the organization of filamentous (F-) actin within individual spines on CA1 pyramidal neurons in rat hippocampal slices. Using two-photon photoactivation of green fluorescent protein fused to [beta]-actin, we found that a dynamic pool of F-actin at the tip of the spine quickly treadmilled to generate an expansive force. The size of a stable F-actin pool at the base of the spine depended on spine volume. Repeated two-photon uncaging of glutamate formed a third pool of F-actin and enlarged the spine. The spine often released this "enlargement pool" into the dendritic shaft, but the pool had to be physically confined by a spine neck for the enlargement to be long-lasting. Ca2+/calmodulin-dependent protein kinase II regulated this confinement. Thus, spines have an elaborate mechanical nature that is regulated by actin fibers. KW - actin KW - cytosqueleton KW - dendritic_spines KW - photoactivation AU - Honkura, Naoki AU - Matsuzaki, Masanori AU - Noguchi, Jun AU - Ellis-Davies, Graham AU - Kasai, Haruo PY - 2008/03/13/ UR - http://dx.doi.org/10.1016/j.neuron.2008.01.013 ER -