Type-1 cannabinoid receptors colocalize with caveolin-1 in neuronal cells.

@article{citeulike:2619215, abstract = {Type-1 (CB1) and type-2 (CB2) cannabinoid receptors belong to the rhodopsin family of G protein-coupled receptors, and are activated by endogenous lipids termed "endocannabinoids". Recent reports have demonstrated that CB1R, unlike CB2R and other receptors and metabolic enzymes of endocannabinoids, functions in the context of lipid rafts, i.e. plasma membrane microdomains which may be important in modulating signal transduction. Here, we present novel data based on cell subfractionation, immunoprecipitation and confocal microscopy studies, that show that in C6 cells CB1R co-localizes almost entirely with caveolin-1. We also show that trafficking of CB1R in response to the raft disruptor methyl-beta-cyclodextrin (MCD) is superimposable on that of caveolin-1, and that MCD treatment increases the accessibility of CB1R to its specific antibodies. These findings may be relevant for the manifold CB1R-dependent activities of endocannabinoids, like the regulation of apoptosis and of neurodegenerative diseases.}, address = {Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, 00133 Rome, Italy.}, author = {Bari, M. and Oddi, S. and De Simone, C. and Spagnolo, P. and Gasperi, V. and Battista, N. and Centonze, D. and Maccarrone, M. }, citeulike-article-id = {2619215}, doi = {10.1016/j.neuropharm.2007.06.030}, issn = {0028-3908}, journal = {Neuropharmacology}, keywords = {caveolin, cb1, endocytosis}, month = {January}, number = {1}, pages = {45--50}, posted-at = {2008-04-01 11:21:25}, priority = {0}, title = {Type-1 cannabinoid receptors colocalize with caveolin-1 in neuronal cells.}, url = {http://dx.doi.org/10.1016/j.neuropharm.2007.06.030}, volume = {54}, year = {2008} }

TY - JOUR ID - citeulike:2619215 L3 - citeulike-article-id:2619215 TI - Type-1 cannabinoid receptors colocalize with caveolin-1 in neuronal cells. JF - Neuropharmacology VL - 54 IS - 1 SP - 45 EP - 50 AD - Department of Experimental Medicine and Biochemical Sciences, University of Rome Tor Vergata, 00133 Rome, Italy. SN - 0028-3908 N2 - Type-1 (CB1) and type-2 (CB2) cannabinoid receptors belong to the rhodopsin family of G protein-coupled receptors, and are activated by endogenous lipids termed "endocannabinoids". Recent reports have demonstrated that CB1R, unlike CB2R and other receptors and metabolic enzymes of endocannabinoids, functions in the context of lipid rafts, i.e. plasma membrane microdomains which may be important in modulating signal transduction. Here, we present novel data based on cell subfractionation, immunoprecipitation and confocal microscopy studies, that show that in C6 cells CB1R co-localizes almost entirely with caveolin-1. We also show that trafficking of CB1R in response to the raft disruptor methyl-beta-cyclodextrin (MCD) is superimposable on that of caveolin-1, and that MCD treatment increases the accessibility of CB1R to its specific antibodies. These findings may be relevant for the manifold CB1R-dependent activities of endocannabinoids, like the regulation of apoptosis and of neurodegenerative diseases. KW - caveolin KW - cb1 KW - endocytosis AU - Bari, M AU - Oddi, S AU - De Simone, C AU - Spagnolo, P AU - Gasperi, V AU - Battista, N AU - Centonze, D AU - Maccarrone, M PY - 2008/01// UR - http://dx.doi.org/10.1016/j.neuropharm.2007.06.030 ER -