Recent advances using green and red fluorescent protein variants.

by: A Müller-Taubenberger, KI Anderson

Appl Microbiol Biotechnol, Vol. 77, No. 1. (November 2007), pp. 1-12.

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Abstract

Fluorescent proteins have proven to be excellent tools for live-cell imaging. In addition to green fluorescent protein (GFP) and its variants, recent progress has led to the development of monomeric red fluorescent proteins (mRFPs) that show improved properties with respect to maturation, brightness, and the monomeric state. This review considers green and red spectral variants, their paired use for live-cell imaging in vivo, in vitro, and in fluorescence resonance energy transfer (FRET) studies, in addition to other recent "two-color" advances including photoswitching and bimolecular fluorescence complementation (BiFC). It will be seen that green and red fluorescent proteins now exist with nearly ideal properties for dual-color microscopy and FRET.

@article{citeulike:2569990, abstract = {Fluorescent proteins have proven to be excellent tools for live-cell imaging. In addition to green fluorescent protein (GFP) and its variants, recent progress has led to the development of monomeric red fluorescent proteins (mRFPs) that show improved properties with respect to maturation, brightness, and the monomeric state. This review considers green and red spectral variants, their paired use for live-cell imaging in vivo, in vitro, and in fluorescence resonance energy transfer (FRET) studies, in addition to other recent "two-color" advances including photoswitching and bimolecular fluorescence complementation (BiFC). It will be seen that green and red fluorescent proteins now exist with nearly ideal properties for dual-color microscopy and FRET.}, address = {Institut f\"{u}r Zellbiologie (ABI), Ludwig-Maximilians-Universit\"{a}t M\"{u}nchen, Schillerstrasse 42, Munich, Germany. amueller@lrz.uni-muenchen.de}, author = {M\"{u}ller-Taubenberger, A. and Anderson, K. I. }, citeulike-article-id = {2569990}, doi = {10.1007/s00253-007-1131-5}, issn = {0175-7598}, journal = {Appl Microbiol Biotechnol}, keywords = {fluorescent\_proteins, review}, month = {November}, number = {1}, pages = {1--12}, posted-at = {2008-03-21 14:32:36}, priority = {0}, title = {Recent advances using green and red fluorescent protein variants.}, url = {http://dx.doi.org/10.1007/s00253-007-1131-5}, volume = {77}, year = {2007} }

RIS record

TY - JOUR ID - citeulike:2569990 L3 - citeulike-article-id:2569990 TI - Recent advances using green and red fluorescent protein variants. JF - Appl Microbiol Biotechnol VL - 77 IS - 1 SP - 1 EP - 12 AD - Institut für Zellbiologie (ABI), Ludwig-Maximilians-Universität München, Schillerstrasse 42, Munich, Germany. amueller@lrz.uni-muenchen.de SN - 0175-7598 N2 - Fluorescent proteins have proven to be excellent tools for live-cell imaging. In addition to green fluorescent protein (GFP) and its variants, recent progress has led to the development of monomeric red fluorescent proteins (mRFPs) that show improved properties with respect to maturation, brightness, and the monomeric state. This review considers green and red spectral variants, their paired use for live-cell imaging in vivo, in vitro, and in fluorescence resonance energy transfer (FRET) studies, in addition to other recent "two-color" advances including photoswitching and bimolecular fluorescence complementation (BiFC). It will be seen that green and red fluorescent proteins now exist with nearly ideal properties for dual-color microscopy and FRET. KW - fluorescent_proteins KW - review AU - Müller-Taubenberger, A AU - Anderson, KI PY - 2007/11// UR - http://dx.doi.org/10.1007/s00253-007-1131-5 ER -

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