MAP kinase pathway-dependent phosphorylation of the L1-CAM ankyrin binding site regulates neuronal growth.

@article{citeulike:2426025, abstract = {The growth of neuronal processes depends critically on the function of adhesion proteins that link extracellular ligands to the cytoskeleton. The neuronal adhesion protein L1-CAM serves as a receptor for nerve growth-promoting proteins, a process that is inhibited by the interaction between L1-CAM and the cytoskeleton adaptor ankyrin. Using a novel reporter based on intramolecular bioluminescence resonance energy transfer, we have determined that the MAP kinase pathway regulates the phosphorylation of the FIGQY motif in the adhesion protein L1-CAM and its interaction with ankyrin B. MAP kinase pathway inhibitors block L1-CAM-mediated neuronal growth. However, this blockade is partially rescued by inhibitors of L1-CAM-ankyrin binding. These results demonstrate that the MAP kinase pathway regulates L1-CAM-mediated nerve growth by modulating ankyrin binding, suggesting that nerve growth can be regulated at the level of individual receptors.}, address = {Department of Pharmacology and Biological Chemistry, Mt. Sinai School of Medicine, New York, NY 10029, USA.}, author = {Whittard, J. D. and Sakurai, T. and Cassella, M. R. and Gazdoiu, M. and Felsenfeld, D. P. }, citeulike-article-id = {2426025}, doi = {10.1091/mbc.E06-01-0090}, issn = {1059-1524}, journal = {Mol Biol Cell}, keywords = {ankyrin, l1, neurites\_growth, phosphorylation}, month = {June}, number = {6}, pages = {2696--2706}, posted-at = {2008-02-25 16:47:36}, priority = {0}, title = {MAP kinase pathway-dependent phosphorylation of the L1-CAM ankyrin binding site regulates neuronal growth.}, url = {http://dx.doi.org/10.1091/mbc.E06-01-0090}, volume = {17}, year = {2006} }

TY - JOUR ID - citeulike:2426025 L3 - citeulike-article-id:2426025 TI - MAP kinase pathway-dependent phosphorylation of the L1-CAM ankyrin binding site regulates neuronal growth. JF - Mol Biol Cell VL - 17 IS - 6 SP - 2696 EP - 2706 AD - Department of Pharmacology and Biological Chemistry, Mt. Sinai School of Medicine, New York, NY 10029, USA. SN - 1059-1524 N2 - The growth of neuronal processes depends critically on the function of adhesion proteins that link extracellular ligands to the cytoskeleton. The neuronal adhesion protein L1-CAM serves as a receptor for nerve growth-promoting proteins, a process that is inhibited by the interaction between L1-CAM and the cytoskeleton adaptor ankyrin. Using a novel reporter based on intramolecular bioluminescence resonance energy transfer, we have determined that the MAP kinase pathway regulates the phosphorylation of the FIGQY motif in the adhesion protein L1-CAM and its interaction with ankyrin B. MAP kinase pathway inhibitors block L1-CAM-mediated neuronal growth. However, this blockade is partially rescued by inhibitors of L1-CAM-ankyrin binding. These results demonstrate that the MAP kinase pathway regulates L1-CAM-mediated nerve growth by modulating ankyrin binding, suggesting that nerve growth can be regulated at the level of individual receptors. KW - ankyrin KW - l1 KW - neurites_growth KW - phosphorylation AU - Whittard, JD AU - Sakurai, T AU - Cassella, MR AU - Gazdoiu, M AU - Felsenfeld, DP PY - 2006/06// UR - http://dx.doi.org/10.1091/mbc.E06-01-0090 ER -