A vital role of tubulin-tyrosine-ligase for neuronal organization.

@article{citeulike:2191373, abstract = {Tubulin is subject to a special cycle of detyrosination/tyrosination in which the C-terminal tyrosine of alpha-tubulin is cyclically removed by a carboxypeptidase and readded by a tubulin-tyrosine-ligase (TTL). This tyrosination cycle is conserved in evolution, yet its physiological importance is unknown. Here, we find that TTL suppression in mice causes perinatal death. A minor pool of tyrosinated (Tyr-)tubulin persists in TTL null tissues, being present mainly in dividing TTL null cells where it originates from tubulin synthesis, but it is lacking in postmitotic TTL null cells such as neurons, which is apparently deleterious because early death in TTL null mice is, at least in part, accounted for by a disorganization of neuronal networks, including a disruption of the cortico-thalamic loop. Correlatively, cultured TTL null neurons display morphogenetic anomalies including an accelerated and erratic time course of neurite outgrowth and a premature axonal differentiation. These anomalies may involve a mislocalization of CLIP170, which we find lacking in neurite extensions and growth cones of TTL null neurons. Our results demonstrate a vital role of TTL for neuronal organization and suggest a requirement of Tyr-tubulin for proper control of neurite extensions.}, address = {Department of Cell Biology, German Research Center for Biotechnology, D-38124 Braunschweig, Germany.}, author = {Erck, C. and Peris, L. and Andrieux, A. and Meissirel, C. and Gruber, A. D. and Vernet, M. and Schweitzer, A. and Saoudi, Y. and Pointu, H. and Bosc, C. and Salin, P. A. and Job, D. and Wehland, J. }, citeulike-article-id = {2191373}, doi = {10.1073/pnas.0409626102}, issn = {0027-8424}, journal = {Proc Natl Acad Sci U S A}, keywords = {cytosqueleton, microtubules, neurites\_growth, neurons, tips, transgenic}, month = {May}, number = {22}, pages = {7853--7858}, posted-at = {2008-01-03 13:09:14}, priority = {0}, title = {A vital role of tubulin-tyrosine-ligase for neuronal organization.}, url = {http://dx.doi.org/10.1073/pnas.0409626102}, volume = {102}, year = {2005} }

TY - JOUR ID - citeulike:2191373 L3 - citeulike-article-id:2191373 TI - A vital role of tubulin-tyrosine-ligase for neuronal organization. JF - Proc Natl Acad Sci U S A VL - 102 IS - 22 SP - 7853 EP - 7858 AD - Department of Cell Biology, German Research Center for Biotechnology, D-38124 Braunschweig, Germany. SN - 0027-8424 N2 - Tubulin is subject to a special cycle of detyrosination/tyrosination in which the C-terminal tyrosine of alpha-tubulin is cyclically removed by a carboxypeptidase and readded by a tubulin-tyrosine-ligase (TTL). This tyrosination cycle is conserved in evolution, yet its physiological importance is unknown. Here, we find that TTL suppression in mice causes perinatal death. A minor pool of tyrosinated (Tyr-)tubulin persists in TTL null tissues, being present mainly in dividing TTL null cells where it originates from tubulin synthesis, but it is lacking in postmitotic TTL null cells such as neurons, which is apparently deleterious because early death in TTL null mice is, at least in part, accounted for by a disorganization of neuronal networks, including a disruption of the cortico-thalamic loop. Correlatively, cultured TTL null neurons display morphogenetic anomalies including an accelerated and erratic time course of neurite outgrowth and a premature axonal differentiation. These anomalies may involve a mislocalization of CLIP170, which we find lacking in neurite extensions and growth cones of TTL null neurons. Our results demonstrate a vital role of TTL for neuronal organization and suggest a requirement of Tyr-tubulin for proper control of neurite extensions. KW - cytosqueleton KW - microtubules KW - neurites_growth KW - neurons KW - tips KW - transgenic AU - Erck, C AU - Peris, L AU - Andrieux, A AU - Meissirel, C AU - Gruber, AD AU - Vernet, M AU - Schweitzer, A AU - Saoudi, Y AU - Pointu, H AU - Bosc, C AU - Salin, PA AU - Job, D AU - Wehland, J PY - 2005/05/31/ UR - http://dx.doi.org/10.1073/pnas.0409626102 ER -