Postsynaptic Positioning of Endocytic Zones and AMPA Receptor Cycling by Physical Coupling of Dynamin-3 to Homer

@article{citeulike:2108896, abstract = {Summary Endocytosis of AMPA receptors and other postsynaptic cargo occurs at endocytic zones (EZs), stably positioned sites of clathrin adjacent to the postsynaptic density (PSD). The tight localization of postsynaptic endocytosis is thought to control spine composition and regulate synaptic transmission. However, the mechanisms that situate the EZ near the PSD and the role of spine endocytosis in synaptic transmission are unknown. Here, we report that a physical link between dynamin-3 and the postsynaptic adaptor Homer positions the EZ near the PSD. Disruption of dynamin-3 or its interaction with Homer uncouples the PSD from the EZ, resulting in synapses lacking postsynaptic clathrin. Loss of the EZ leads to a loss of synaptic AMPA receptors and reduced excitatory synaptic transmission that corresponds with impaired synaptic recycling. Thus, a physical link between the PSD and the EZ ensures localized endocytosis and recycling by recapturing and maintaining a proximate pool of cycling AMPA receptors.}, author = {Lu, Jiuyi and Helton, Thomas D. and Blanpied, Thomas A. and Racz, Bence and Newpher, Thomas M. and Weinberg, Richard J. and Ehlers, Michael D. }, citeulike-article-id = {2108896}, doi = {10.1016/j.neuron.2007.06.041}, journal = {Neuron}, keywords = {ampar, cultured\_hippo\_neurons, dendritic\_spines, endocytosis, synapse}, month = {September}, number = {6}, pages = {874--889}, posted-at = {2008-02-25 16:44:17}, priority = {0}, title = {Postsynaptic Positioning of Endocytic Zones and AMPA Receptor Cycling by Physical Coupling of Dynamin-3 to Homer}, url = {http://dx.doi.org/10.1016/j.neuron.2007.06.041}, volume = {55}, year = {2007} }

TY - JOUR ID - citeulike:2108896 L3 - citeulike-article-id:2108896 TI - Postsynaptic Positioning of Endocytic Zones and AMPA Receptor Cycling by Physical Coupling of Dynamin-3 to Homer JF - Neuron VL - 55 IS - 6 SP - 874 EP - 889 N2 - Summary Endocytosis of AMPA receptors and other postsynaptic cargo occurs at endocytic zones (EZs), stably positioned sites of clathrin adjacent to the postsynaptic density (PSD). The tight localization of postsynaptic endocytosis is thought to control spine composition and regulate synaptic transmission. However, the mechanisms that situate the EZ near the PSD and the role of spine endocytosis in synaptic transmission are unknown. Here, we report that a physical link between dynamin-3 and the postsynaptic adaptor Homer positions the EZ near the PSD. Disruption of dynamin-3 or its interaction with Homer uncouples the PSD from the EZ, resulting in synapses lacking postsynaptic clathrin. Loss of the EZ leads to a loss of synaptic AMPA receptors and reduced excitatory synaptic transmission that corresponds with impaired synaptic recycling. Thus, a physical link between the PSD and the EZ ensures localized endocytosis and recycling by recapturing and maintaining a proximate pool of cycling AMPA receptors. KW - ampar KW - cultured_hippo_neurons KW - dendritic_spines KW - endocytosis KW - synapse AU - Lu, Jiuyi AU - Helton, Thomas AU - Blanpied, Thomas AU - Racz, Bence AU - Newpher, Thomas AU - Weinberg, Richard AU - Ehlers, Michael PY - 2007/09/20/ UR - http://dx.doi.org/10.1016/j.neuron.2007.06.041 ER -