SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment.

@article{citeulike:1913019, abstract = {SNX-BAR proteins are a sub-family of sorting nexins implicated in endosomal sorting. Here, we establish that through its phox homology (PX) and Bin-Amphiphysin-Rvs (BAR) domains, sorting nexin-4 (SNX4) is associated with tubular and vesicular elements of a compartment that overlaps with peripheral early endosomes and the juxtanuclear endocytic recycling compartment (ERC). Suppression of SNX4 perturbs transport between these compartments and causes lysosomal degradation of the transferrin receptor (TfnR). Through an interaction with KIBRA, a protein previously shown to bind dynein light chain 1, we establish that SNX4 associates with the minus end-directed microtubule motor dynein. Although suppression of KIBRA and dynein perturbs early endosome-to-ERC transport, TfnR sorting is maintained. We propose that by driving membrane tubulation, SNX4 coordinates iterative, geometric-based sorting of the TfnR with the long-range transport of carriers from early endosomes to the ERC. Finally, these data suggest that by associating with molecular motors, SNX-BAR proteins may coordinate sorting with carrier transport between donor and recipient membranes.}, address = {The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK.}, author = {Traer, Colin J J. and Rutherford, Anna C C. and Palmer, Krysten J J. and Wassmer, Thomas and Oakley, Jacqueline and Attar, Naomi and Carlton, Jez G G. and Kremerskothen, Joachim and Stephens, David J J. and Cullen, Peter J J. }, citeulike-article-id = {1913019}, comment = {SNX4 has a PX and BAR domain, geometric sorting from sorting endosome to endocytic recycling compartment. Coupled with KIBRA and dynein. Recycling to the PM mediated by KIF16B Sorting endosome to Golgi is depedent on SNX1}, doi = {10.1038/ncb1656}, issn = {1465-7392}, journal = {Nat Cell Biol}, keywords = {cell\_culture, endocytosis, endosomes, recycling}, month = {November}, posted-at = {2008-01-03 10:08:45}, priority = {0}, title = {SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment.}, url = {http://dx.doi.org/10.1038/ncb1656}, year = {2007} }

TY - JOUR ID - citeulike:1913019 L3 - citeulike-article-id:1913019 TI - SNX4 coordinates endosomal sorting of TfnR with dynein-mediated transport into the endocytic recycling compartment. JF - Nat Cell Biol AD - The Henry Wellcome Integrated Signalling Laboratories, Department of Biochemistry, School of Medical Sciences, University of Bristol, Bristol BS8 1TD, UK. SN - 1465-7392 N2 - SNX-BAR proteins are a sub-family of sorting nexins implicated in endosomal sorting. Here, we establish that through its phox homology (PX) and Bin-Amphiphysin-Rvs (BAR) domains, sorting nexin-4 (SNX4) is associated with tubular and vesicular elements of a compartment that overlaps with peripheral early endosomes and the juxtanuclear endocytic recycling compartment (ERC). Suppression of SNX4 perturbs transport between these compartments and causes lysosomal degradation of the transferrin receptor (TfnR). Through an interaction with KIBRA, a protein previously shown to bind dynein light chain 1, we establish that SNX4 associates with the minus end-directed microtubule motor dynein. Although suppression of KIBRA and dynein perturbs early endosome-to-ERC transport, TfnR sorting is maintained. We propose that by driving membrane tubulation, SNX4 coordinates iterative, geometric-based sorting of the TfnR with the long-range transport of carriers from early endosomes to the ERC. Finally, these data suggest that by associating with molecular motors, SNX-BAR proteins may coordinate sorting with carrier transport between donor and recipient membranes. KW - cell_culture KW - endocytosis KW - endosomes KW - recycling N1 - SNX4 has a PX and BAR domain, geometric sorting from sorting endosome to endocytic recycling compartment. Coupled with KIBRA and dynein. Recycling to the PM mediated by KIF16B Sorting endosome to Golgi is depedent on SNX1 AU - Traer, Colin J AU - Rutherford, Anna C AU - Palmer, Krysten J AU - Wassmer, Thomas AU - Oakley, Jacqueline AU - Attar, Naomi AU - Carlton, Jez G AU - Kremerskothen, Joachim AU - Stephens, David J AU - Cullen, Peter J PY - 2007/11/11/ UR - http://dx.doi.org/10.1038/ncb1656 ER -