Endocytosis Optimizes the Dynamic Localization of Membrane Proteins that Regulate Cortical Polarity

@article{citeulike:1255559, abstract = {Summary Diverse cell types require the ability to maintain dynamically polarized membrane-protein distributions through balancing transport and diffusion. However, design principles underlying dynamically maintained cortical polarity are not well understood. Here we constructed a mathematical model for characterizing the morphology of dynamically polarized protein distributions. We developed analytical approaches for measuring all model parameters from single-cell experiments. We applied our methods to a well-characterized system for studying polarized membrane proteins: budding yeast cells expressing activated Cdc42. We found that a balance of diffusion, directed transport, and endocytosis was sufficient for accurately describing polarization morphologies. Surprisingly, the model predicts that polarized regions are defined with a precision that is nearly optimal for measured endocytosis rates and that polarity can be dynamically stabilized through positive feedback with directed transport. Our approach provides a step toward understanding how biological systems shape spatially precise, unambiguous cortical polarity domains using dynamic processes.}, author = {Marco, Eugenio and Wedlich-Soldner, Roland and Li, Rong and Altschuler, Steven J. and Wu, Lani F. }, citeulike-article-id = {1255559}, journal = {Cell}, keywords = {endocytosis, frap, membrane\_diffusion, membrane\_insertion, model, polarity}, month = {April}, number = {2}, pages = {411--422}, posted-at = {2007-04-26 11:13:46}, priority = {0}, title = {Endocytosis Optimizes the Dynamic Localization of Membrane Proteins that Regulate Cortical Polarity}, url = {http://www.sciencedirect.com/science/article/B6WSN-4NHMXD1-R/2/69129ed4e69c1d9017ddadc5f982d012}, volume = {129}, year = {2007} }

TY - JOUR ID - citeulike:1255559 L3 - citeulike-article-id:1255559 TI - Endocytosis Optimizes the Dynamic Localization of Membrane Proteins that Regulate Cortical Polarity JF - Cell VL - 129 IS - 2 SP - 411 EP - 422 N2 - Summary Diverse cell types require the ability to maintain dynamically polarized membrane-protein distributions through balancing transport and diffusion. However, design principles underlying dynamically maintained cortical polarity are not well understood. Here we constructed a mathematical model for characterizing the morphology of dynamically polarized protein distributions. We developed analytical approaches for measuring all model parameters from single-cell experiments. We applied our methods to a well-characterized system for studying polarized membrane proteins: budding yeast cells expressing activated Cdc42. We found that a balance of diffusion, directed transport, and endocytosis was sufficient for accurately describing polarization morphologies. Surprisingly, the model predicts that polarized regions are defined with a precision that is nearly optimal for measured endocytosis rates and that polarity can be dynamically stabilized through positive feedback with directed transport. Our approach provides a step toward understanding how biological systems shape spatially precise, unambiguous cortical polarity domains using dynamic processes. KW - endocytosis KW - frap KW - membrane_diffusion KW - membrane_insertion KW - model KW - polarity AU - Marco, Eugenio AU - Wedlich-Soldner, Roland AU - Li, Rong AU - Altschuler, Steven AU - Wu, Lani PY - 2007/04/20/ UR - http://www.sciencedirect.com/science/article/B6WSN-4NHMXD1-R/2/69129ed4e69c1d9017ddadc5f982d012 ER -